Mass spectrometric analysis of human transferrin in different body fluids.

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Version: author
Serval ID
serval:BIB_815E14CA1271
Type
Article: article from journal or magazin.
Collection
Publications
Title
Mass spectrometric analysis of human transferrin in different body fluids.
Journal
Clinical Chemistry and Laboratory Medicine
Author(s)
Kleinert P., Kuster T., Durka S., Ballhausen D., Bosshard N.U., Steinmann B., Hänseler E., Jaeken J., Heizmann C.W., Troxler H.
ISSN
1434-6621 (Print)
ISSN-L
1434-6621
Publication state
Published
Issued date
2003
Volume
41
Number
12
Pages
1580-1588
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Abstract
In this study, we present a versatile new procedure for the analysis of transferrin and its isoforms isolated from human body fluids such as serum, plasma, and cerebrospinal fluid. This method is based on a three-step procedure: (i) isolation of transferrins using anion-exchange chromatography with UV detection; (ii) concentration of the transferrin fraction; (iii) detection of the transferrins with liquid chromatography-electrospray mass spectrometry. Pre-analytical sample procedures can be omitted and no immunoaffinity columns or transferrin-specific immunoassays were used. Anticoagulants such as heparin, EDTA, citrate, and oxalate do not interfere with our analysis. According to their respective molecular masses, up to ten different isoforms of transferrin could be identified in a serum sample from a patient with a congenital disorder of glycosylation type Ia (CDG-Ia). The method was successfully applied to different pathological samples from patients with CDG-Ia, CDG-Ib, CDG-Ic, CDG-Ie, CDG-If, and CDG-IIa. Additionally, samples from alcohol consumers that were found with turbidimetric immunoassay to contain increased levels of carbohydrate-deficient transferrin were analyzed.
Keywords
Body Fluids/chemistry, Carbohydrate Metabolism, Inborn Errors/diagnosis, Chromatography, Liquid, Humans, Protein Isoforms/analysis, Spectrometry, Mass, Electrospray Ionization/methods, Transferrin/analysis, Transferrin/isolation & purification
Pubmed
Web of science
Create date
29/03/2010 12:21
Last modification date
20/08/2019 14:41
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