Mass spectrometric analysis of human transferrin in different body fluids.

Détails

Ressource 1Demande d'une copieEtat: Supprimée
Version: de l'auteur
ID Serval
serval:BIB_815E14CA1271
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Mass spectrometric analysis of human transferrin in different body fluids.
Périodique
Clinical Chemistry and Laboratory Medicine
Auteur(s)
Kleinert P., Kuster T., Durka S., Ballhausen D., Bosshard N.U., Steinmann B., Hänseler E., Jaeken J., Heizmann C.W., Troxler H.
ISSN
1434-6621 (Print)
ISSN-L
1434-6621
Statut éditorial
Publié
Date de publication
2003
Volume
41
Numéro
12
Pages
1580-1588
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Résumé
In this study, we present a versatile new procedure for the analysis of transferrin and its isoforms isolated from human body fluids such as serum, plasma, and cerebrospinal fluid. This method is based on a three-step procedure: (i) isolation of transferrins using anion-exchange chromatography with UV detection; (ii) concentration of the transferrin fraction; (iii) detection of the transferrins with liquid chromatography-electrospray mass spectrometry. Pre-analytical sample procedures can be omitted and no immunoaffinity columns or transferrin-specific immunoassays were used. Anticoagulants such as heparin, EDTA, citrate, and oxalate do not interfere with our analysis. According to their respective molecular masses, up to ten different isoforms of transferrin could be identified in a serum sample from a patient with a congenital disorder of glycosylation type Ia (CDG-Ia). The method was successfully applied to different pathological samples from patients with CDG-Ia, CDG-Ib, CDG-Ic, CDG-Ie, CDG-If, and CDG-IIa. Additionally, samples from alcohol consumers that were found with turbidimetric immunoassay to contain increased levels of carbohydrate-deficient transferrin were analyzed.
Mots-clé
Body Fluids/chemistry, Carbohydrate Metabolism, Inborn Errors/diagnosis, Chromatography, Liquid, Humans, Protein Isoforms/analysis, Spectrometry, Mass, Electrospray Ionization/methods, Transferrin/analysis, Transferrin/isolation & purification
Pubmed
Web of science
Création de la notice
29/03/2010 13:21
Dernière modification de la notice
03/03/2018 18:47
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