Molecular evolution of Azagny virus, a newfound hantavirus harbored by the West African pygmy shrew (Crocidura obscurior) in Côte d'Ivoire.

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ID Serval
serval:BIB_7FFCDD0A37CD
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Molecular evolution of Azagny virus, a newfound hantavirus harbored by the West African pygmy shrew (Crocidura obscurior) in Côte d'Ivoire.
Périodique
Virology Journal
Auteur(s)
Kang H.J., Kadjo B., Dubey S., Jacquet F., Yanagihara R.
ISSN
1743-422X (Electronic)
ISSN-L
1743-422X
Statut éditorial
Publié
Date de publication
2011
Volume
8
Pages
373
Langue
anglais
Résumé
BACKGROUND:Tanganya virus (TGNV), the only shrew-associated hantavirus reported to date from sub-Saharan Africa, is harbored by the Therese's shrew (Crocidura theresae), and is phylogenetically distinct from Thottapalayam virus (TPMV) in the Asian house shrew (Suncus murinus) and Imjin virus (MJNV) in the Ussuri white-toothed shrew (Crocidura lasiura). The existence of myriad soricid-borne hantaviruses in Eurasia and North America would predict the presence of additional hantaviruses in sub-Saharan Africa, where multiple shrew lineages have evolved and diversified.METHODS:Lung tissues, collected in RNAlater®, from 39 Buettikofer's shrews (Crocidura buettikoferi), 5 Jouvenet's shrews (Crocidura jouvenetae), 9 West African pygmy shrews (Crocidura obscurior) and 21 African giant shrews (Crocidura olivieri) captured in Côte d'Ivoire during 2009, were systematically examined for hantavirus RNA by RT-PCR.RESULTS:A genetically distinct hantavirus, designated Azagny virus (AZGV), was detected in the West African pygmy shrew. Phylogenetic analysis of the S, M and L segments, using maximum-likelihood and Bayesian methods, under the GTR+I+Γ model of evolution, showed that AZGV shared a common ancestry with TGNV and was more closely related to hantaviruses harbored by soricine shrews than to TPMV and MJNV. That is, AZGV in the West African pygmy shrew, like TGNV in the Therese's shrew, did not form a monophyletic group with TPMV and MJNV, which were deeply divergent and basal to other rodent- and soricomorph-borne hantaviruses. Ancestral distributions of each hantavirus lineage, reconstructed using Mesquite 2.74, suggested that the common ancestor of all hantaviruses was most likely of Eurasian, not African, origin.CONCLUSIONS:Genome-wide analysis of many more hantaviruses from sub-Saharan Africa are required to better understand how the biogeographic origin and radiation of African shrews might have contributed to, or have resulted from, the evolution of hantaviruses.
Mots-clé
Animals, Cluster Analysis, Type="Geographic">Cote d'Ivoire, Evolution, Molecular, Genotype, Hantavirus/classification, Hantavirus/genetics, Hantavirus Infections/veterinary, Hantavirus Infections/virology, Insectivora/virology, Lung/virology, Molecular Sequence Data, Phylogeny, RNA, Viral/genetics, Sequence Analysis, DNA
Pubmed
Web of science
Open Access
Oui
Création de la notice
18/10/2011 12:21
Dernière modification de la notice
20/08/2019 14:40
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