Monocytes from patients with diabetic retinopathy are characterized by increased VEGF transcription during early macrophage differentiation
Details
Serval ID
serval:BIB_7FF8E303B5CE
Type
Inproceedings: an article in a conference proceedings.
Publication sub-type
Abstract (Abstract): shot summary in a article that contain essentials elements presented during a scientific conference, lecture or from a poster.
Collection
Publications
Institution
Title
Monocytes from patients with diabetic retinopathy are characterized by increased VEGF transcription during early macrophage differentiation
Title of the conference
2017 ARVO Annual Meeting. Baltimora, MD, USA
Organization
2017 ARVO Annual Meeting, held in Baltimore, MD,
Address
2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.
Publication state
Published
Issued date
07/05/2017
Peer-reviewed
Oui
Volume
58
Pages
2529
Language
english
Notes
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 2529
Abstract
Purpose : Type 2 diabetes (T2DM) affects a growing part of the population in all developed countries. More than 60% of people with T2DM will develop a form a Diabetic Retinopathy (DR) after 20 years of diabetes. Diabetic patients demonstrate robustly elevated intravitreal levels of inflammatory cytokines. Infiltrating monocyte (Mo)-derived inflammatory Macrophages (MPs) account for most of the production of these cytokines in animal models of DR. We hypothesized that pre-activated circulating inflammatory monocytes found in T2DM are an important pathogenic factor in DR.
Methods : T2DM and control patients were enrolled and diagnosed for diabetic retinopathy. Four groups were defined as follows: (i) controls, (ii) diabetic patients with no retinopathy, (iii) patients with NPDR, and (iv) PDR patients. Patients with known history of nephropathy and periodontitis were excluded. Plasma and monocytes were collected from all groups. Fresh monocytes were allowed to differentiate into MPs for 18h and RNA and culture supernatant were collected. Fresh Monocytes and monocyte-derived MPs were then evaluated for their ability to produce cytokines known to be elevated in the vitreous of DR patients including IL-1β, IL-6, IL-8, CCL2, and VEGF
Results : Seventy-two subjects were included. The 3 diabetic groups were evenly distributed for age, BMI, blood glucose and Hba1c. Circulating monocytes demonstrated low expression of cytokines with minor differences between groups. In contrast, upon differentiation MP increased their expression of all tested cytokines. After 18h the expression of VEGF was higher in patients with NPDR or PDR than in diabetic patients with no retinopathy.
Conclusions : Our data support a model in which circulating monocytes in DR patients have a greater propensity to produce inflammatory cytokines when they differentiate into inflammatory macrophages compared to T2DM patients. Understanding how monocyte derived-inflammatory cytokine influence DR and treatment outcomes might be new cues to propose innovative treatments of these pathologies.
This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.
Methods : T2DM and control patients were enrolled and diagnosed for diabetic retinopathy. Four groups were defined as follows: (i) controls, (ii) diabetic patients with no retinopathy, (iii) patients with NPDR, and (iv) PDR patients. Patients with known history of nephropathy and periodontitis were excluded. Plasma and monocytes were collected from all groups. Fresh monocytes were allowed to differentiate into MPs for 18h and RNA and culture supernatant were collected. Fresh Monocytes and monocyte-derived MPs were then evaluated for their ability to produce cytokines known to be elevated in the vitreous of DR patients including IL-1β, IL-6, IL-8, CCL2, and VEGF
Results : Seventy-two subjects were included. The 3 diabetic groups were evenly distributed for age, BMI, blood glucose and Hba1c. Circulating monocytes demonstrated low expression of cytokines with minor differences between groups. In contrast, upon differentiation MP increased their expression of all tested cytokines. After 18h the expression of VEGF was higher in patients with NPDR or PDR than in diabetic patients with no retinopathy.
Conclusions : Our data support a model in which circulating monocytes in DR patients have a greater propensity to produce inflammatory cytokines when they differentiate into inflammatory macrophages compared to T2DM patients. Understanding how monocyte derived-inflammatory cytokine influence DR and treatment outcomes might be new cues to propose innovative treatments of these pathologies.
This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.
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