Separation and characterization of late endosomal membrane domains.

Détails

ID Serval
serval:BIB_7F59603370AC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Separation and characterization of late endosomal membrane domains.
Périodique
Journal of Biological Chemistry
Auteur(s)
Kobayashi T., Beuchat M.H., Chevallier J., Makino A., Mayran N., Escola J.M., Lebrand C., Cosson P., Kobayashi T., Gruenberg J.
ISSN
0021-9258
Statut éditorial
Publié
Date de publication
08/2002
Peer-reviewed
Oui
Volume
277
Numéro
35
Pages
32157-32164
Langue
anglais
Résumé
Very little is known about the biophysical properties and the lipid or protein composition of membrane domains presumably present in endocytic and biosynthetic organelles. Here we analyzed the membrane composition of late endosomes by suborganellar fractionation in the absence of detergent. We found that the internal membranes of this multivesicular organelle can be separated from the limiting membrane and that each membrane population exhibited a defined composition. Our data also indicated that internal membranes may consist of at least two populations, containing primarily phosphatidylcholine or lysobisphosphatidic acid as major phospholipid, arguing for the existence of significant microheterogeneity within late endosomal membranes. We also found that lysobisphosphatidic acid exhibited unique pH-dependent fusogenic properties, and we speculated that this lipid is an ideal candidate to regulate the dynamic properties of this internal membrane mosaic.
Mots-clé
Animals, Cell Line, Cricetinae, Endosomes/chemistry, Endosomes/ultrastructure, Fatty Acids, Nonesterified/analysis, Hydrogen-Ion Concentration, Intracellular Membranes/chemistry, Intracellular Membranes/ultrastructure, Kidney, Lysophospholipids/analysis, Membrane Fusion, Membrane Lipids/analysis, Monoglycerides, Phospholipids/analysis
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 15:27
Dernière modification de la notice
08/05/2019 21:03
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