Atrial natriuretic peptide effects on intracellular pH changes and ROS production in HEPG2 cells: role of p38 MAPK and phospholipase D.

Détails

ID Serval
serval:BIB_7F559009B46F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Atrial natriuretic peptide effects on intracellular pH changes and ROS production in HEPG2 cells: role of p38 MAPK and phospholipase D.
Périodique
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology
Auteur(s)
Baldini P.M., De Vito P., Vismara D., Bagni C., Zalfa F., Minieri M., Di Nardo P.
ISSN
1015-8987 (Print)
ISSN-L
1015-8987
Statut éditorial
Publié
Date de publication
2005
Volume
15
Numéro
1-4
Pages
77-88
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
The present study was performed to evaluate Atrial Natriuretic Peptide (ANP) effects on intracellular pH, phospholipase D and ROS production and the possible relationship among them in HepG2 cells. Cancer extracellular microenvironment is more acidic than normal tissues and the activation of NHE-1, the only system able to regulate pHi homeostasis in this condition, can represent an important event in cell proliferation and malignant transformation.
The ANP effects on pHi were evaluated by fluorescence spectrometry. The effects on p38 MAPK and ROS production were evaluated by immunoblots and analysis of DCF-DA fluorescence, respectively. RT-PCR analysis and Western blotting were used to determine the ANP effect on mRNA NHE-1 expression and protein levels. PLD-catalyzed conversion of phosphatidylcholine to phosphatydilethanol (PetOH), in the presence of ethanol, was monitored by thin layer chromatography.
A significant pHi decrease was observed in ANP-treated HepG2 cells and this effect was paralleled by the enhancement of PLD activity and ROS production. The ANP effect on pHi was coupled to an increased p38 MAPK phosphorylation and a down-regulation of mRNA NHE-1 expression and protein levels. Moreover, the relationship between PLD and ROS production was demonstrated by calphostin-c, a potent inhibitor of PLD. At the same time, all assessed ANP-effects were mediated by NPR-C receptors.
Our results indicate that ANP recruits a signal pathway associated with p38 MAPK, NHE-1 and PLD responsible for ROS production, suggesting a possible role for ANP as novel modulator of ROS generation in HepG2 cells.

Mots-clé
Atrial Natriuretic Factor/pharmacology, Cell Line, Tumor, Enzyme Activation/drug effects, Glycerophospholipids/biosynthesis, Glycerophospholipids/metabolism, Humans, Hydrogen-Ion Concentration/drug effects, Immunoblotting, Intracellular Fluid/drug effects, Intracellular Fluid/metabolism, Naphthalenes/pharmacology, Phospholipase D/metabolism, Reactive Oxygen Species/metabolism, Receptors, Atrial Natriuretic Factor/metabolism, p38 Mitogen-Activated Protein Kinases/metabolism
Pubmed
Création de la notice
06/03/2017 18:23
Dernière modification de la notice
03/03/2018 18:43
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