Splenic NK1.1-negative, TCR alpha beta intermediate CD4+ T cells exist in naive NK1.1 allelic positive and negative mice, with the capacity to rapidly secrete large amounts of IL-4 and IFN-gamma upon primary TCR stimulation
Details
Serval ID
serval:BIB_7EC324F76495
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Splenic NK1.1-negative, TCR alpha beta intermediate CD4+ T cells exist in naive NK1.1 allelic positive and negative mice, with the capacity to rapidly secrete large amounts of IL-4 and IFN-gamma upon primary TCR stimulation
Journal
Journal of Immunology
ISSN
0022-1767 (Print)
Publication state
Published
Issued date
05/1999
Volume
162
Number
9
Pages
5156-63
Notes
Journal Article
Research Support, U.S. Gov't, P.H.S. --- Old month value: May 1
Research Support, U.S. Gov't, P.H.S. --- Old month value: May 1
Abstract
Splenic NK1.1+CD4+ T cells that express intermediate levels of TCR alpha beta molecules (TCRint) and the DX5 Ag (believed to identify an equivalent population in NK1.1 allelic negative mice) possess the ability to rapidly produce high quantities of immunomodulatory cytokines, notably IL-4 and IFN-gamma, upon primary TCR activation in vivo. Indeed, only T cells expressing the NK1.1 Ag appear to be capable of this function. In this study, we demonstrate that splenic NK1.1-negative TCRintCD4+ T cells, identified on the basis of Fc gamma R expression, exist in naive NK1.1 allelic positive (C57BL/6) and negative (C3H/HeN) mice with the capacity to produce large amounts of IL-4 and IFN-gamma after only 8 h of primary CD3 stimulation in vitro. Furthermore, a comparison of the amounts of early cytokines produced by Fc gamma R+CD4+TCRint T cells with NK1. 1+CD4+ or DX5+CD4+TCRint T cells, simultaneously isolated from C57BL/6 or C3H/HeN mice, revealed strain and population differences. Thus, Fc gamma R defines another subpopulation of splenic CD4+TCRint cells that can rapidly produce large concentrations of immunomodulatory cytokines, suggesting that CD4+TCRint T cells themselves may represent a unique family of immunoregulatory CD4+ T cells whose members include Fc gamma R+CD4+ and NK1.1/DX5+CD4+ T cells.
Keywords
*Alleles
Animals
Antibodies, Monoclonal/pharmacology
Antigens/*biosynthesis/genetics
Antigens, CD3/immunology
Antigens, Surface
CD4-Positive T-Lymphocytes/*immunology/metabolism
Cells, Cultured
Female
Immunophenotyping
Interferon Type II/biosynthesis/*secretion
Interleukin-4/biosynthesis/*secretion
Lectins, C-Type
*Lymphocyte Activation/genetics/immunology
Mice
Mice, Inbred C3H
Mice, Inbred C57BL
*Protein Biosynthesis
Proteins/genetics
Receptors, Antigen, T-Cell, alpha-beta/*biosynthesis/physiology
Receptors, IgG/biosynthesis
Spleen/cytology/*immunology
T-Lymphocyte Subsets/immunology/metabolism
Pubmed
Web of science
Create date
25/01/2008 16:50
Last modification date
20/08/2019 14:39