Nasal resistome development in infants with cystic fibrosis in the first year of life

Détails

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Etat: Public
Version: Final published version
Licence: CC BY 4.0
ID Serval
serval:BIB_7EA36E4E2B15
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Nasal resistome development in infants with cystic fibrosis in the first year of life
Périodique
Frontiers in Microbiology
Auteur(s)
Allemann A., Kraemer J.G., Korten I., Ramsey K., Casaulta C., Wüthrich D., Ramette A., Endimiani A., Latzin P., Hilty M.
Collaborateur(s)
SCILD Study Group
ISSN
1664-302X (Print)
ISSN-L
1664-302X
Statut éditorial
Publié
Date de publication
2019
Peer-reviewed
Oui
Volume
10
Numéro
212
Pages
[1-14]
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Résumé
Polymicrobial infections of the respiratory tract due to antibiotic resistant bacteria are a great concern in patients with cystic fibrosis (CF). We therefore aimed at establishing a functional metagenomic method to analyze the nasal resistome in infants with CF within the first year of life. We included samples from patients before antibiotic treatment, which allowed obtaining information regarding natural status of the resistome. In total, we analyzed 130 nasal swabs from 26 infants with CF and screened for β-lactams (ampicillin, amoxicillin-clavulanic acid, and cefuroxime) and other classes of antibiotic resistances (tetracycline, chloramphenicol and trimethoprim-sulfamethoxazole). For 69 swabs (53% of total), we found at least one non-susceptible phenotype. Analyses of the inserts recovered from non-susceptible clones by nanopore MinION sequencing revealed a large reservoir of resistance genes including mobile elements within the antibiotic naïve samples. Comparing the data of the resistome with the microbiota composition showed that the bacterial phyla and operational taxonomic units (OTUs) of the microbiota rather than the antibiotic treatment were associated with the majority of non-susceptible phenotypes in the resistome. Future studies will reveal if characterization of the resistome can help in clinical decision-making in patients with CF.
Mots-clé
antibiotics, beta-lactamases, functional metagenomics, nasal swabs, resistance
Pubmed
Web of science
Open Access
Oui
Création de la notice
08/04/2019 17:23
Dernière modification de la notice
20/08/2019 15:39
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