An RNA-dependent and phase-separated active subnuclear compartment safeguards repressive chromatin domains.
Details
Serval ID
serval:BIB_7E94B9835811
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
An RNA-dependent and phase-separated active subnuclear compartment safeguards repressive chromatin domains.
Journal
Molecular cell
ISSN
1097-4164 (Electronic)
ISSN-L
1097-2765
Publication state
In Press
Peer-reviewed
Oui
Language
english
Notes
Publication types: Journal Article
Publication Status: aheadofprint
Publication Status: aheadofprint
Abstract
The nucleus is composed of functionally distinct membraneless compartments that undergo phase separation (PS). However, whether different subnuclear compartments are connected remains elusive. We identified a type of nuclear body with PS features composed of BAZ2A that associates with active chromatin. BAZ2A bodies depend on RNA transcription and BAZ2A non-disordered RNA-binding TAM domain. Although BAZ2A and H3K27me3 occupancies anticorrelate in the linear genome, in the nuclear space, BAZ2A bodies contact H3K27me3 bodies. BAZ2A-body disruption promotes BAZ2A invasion into H3K27me3 domains, causing H3K27me3-body loss and gene upregulation. Weak BAZ2A-RNA interactions, such as with nascent transcripts, promote BAZ2A bodies, whereas the strong binder long non-coding RNA (lncRNA) Malat1 impairs them while mediating BAZ2A association to chromatin at nuclear speckles. In addition to unraveling a direct connection between nuclear active and repressive compartments through PS mechanisms, the results also showed that the strength of RNA-protein interactions regulates this process, contributing to nuclear organization and the regulation of chromatin and gene expression.
Keywords
Baz2a, H3K27me3, Malat1, Rna, chromatin, ground-state pluripotency, nuclear condensates, nuclear speckles, phase separation, BAZ2A, RNA
Pubmed
Open Access
Yes
Create date
12/04/2024 12:16
Last modification date
13/04/2024 7:05