Multiplexed nanoplasmonic biosensor for one-step simultaneous detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine.

Détails

Ressource 1Télécharger: 1-s2.0-S0956566317302130-main.pdf (898.57 [Ko])
Etat: Serval
Version: Final published version
ID Serval
serval:BIB_7E6CA8747A79
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Multiplexed nanoplasmonic biosensor for one-step simultaneous detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine.
Périodique
Biosensors & bioelectronics
Auteur(s)
Soler M., Belushkin A., Cavallini A., Kebbi-Beghdadi C., Greub G., Altug H.
ISSN
1873-4235 (Electronic)
ISSN-L
0956-5663
Statut éditorial
Publié
Date de publication
15/08/2017
Peer-reviewed
Oui
Volume
94
Pages
560-567
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Development of rapid and multiplexed diagnostic tools is a top priority to address the current epidemic problem of sexually transmitted diseases. Here we introduce a novel nanoplasmonic biosensor for simultaneous detection of the two most common bacterial infections: Chlamydia trachomatis and Neisseria gonorrhoeae. Our plasmonic microarray is composed of gold nanohole sensor arrays that exhibit the extraordinary optical transmission (EOT), providing highly sensitive analysis in a label-free configuration. The integration in a microfluidic system and the precise immobilization of specific antibodies on the individual sensor arrays allow for selective detection and quantification of the bacteria in real-time. We achieved outstanding sensitivities for direct immunoassay of urine samples, with a limit of detection of 300 colony forming units (CFU)/mL for C. trachomatis and 1500CFU/mL for N. gonorrhoeae. The multiplexing capability of our biosensor was demonstrated by analyzing different urine samples spiked with either C. trachomatis or N. gonorrhoeae, and also containing both bacteria. We could successfully detect, identify and quantify the levels of the two bacteria in a one-step assay, without the need for DNA extraction or amplification techniques. This work opens up new possibilities for the implementation of point-of-care biosensors that enable fast, simple and efficient diagnosis of sexually transmitted infections.

Mots-clé
Biosensing Techniques/methods, Chlamydia Infections/urine, Chlamydia trachomatis/chemistry, Chlamydia trachomatis/isolation & purification, DNA, Bacterial/chemistry, DNA, Bacterial/isolation & purification, DNA, Bacterial/urine, Gonorrhea/microbiology, Gonorrhea/urine, Humans, Neisseria gonorrhoeae/chemistry, Neisseria gonorrhoeae/isolation & purification, Point-of-Care Systems, Bacteria detection, Diagnosis, Multiplexing, Nanohole array, Nanoplasmonic biosensor, Sexually transmitted infections
Pubmed
Web of science
Open Access
Oui
Création de la notice
11/04/2017 19:00
Dernière modification de la notice
08/05/2019 20:59
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