Article: article from journal or magazin.
Expression of the proto-oncogene c-fos in three-dimensional fetal brain cell cultures and the lack of correlation with maturation-inducing stimuli.
Brain Research. Molecular Brain Research
Previous work has shown that aggregating fetal brain cell cultures are able to attain a highly differentiated state, and that their development is greatly enhanced by growth and/or differentiation factors such as epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), and the protein kinase C-activating tumor promoter mezerein. The present study shows that in these 3-dimensional cultures the peptide growth factors EGF and bFGF as well as mezerein are able to induce the expression of the proto-oncogene c-fos. This induction was rapid and transient, in good agreement with observations reported from a wide variety of cell types in vitro. The maximal levels of c-fos mRNA found after stimulation were low in immature cultures and increased greatly as maturation progressed. Of the three factors tested, mezerein was the most potent inducer of c-fos. In contrast to the peptide growth factors EGF and bFGF which were found to induce c-fos only in glial cells, mezerein was stimulatory in glial cells as well as in neurons. A similar cell type specificity has been observed previously for the maturation-enhancing response in immature aggregate cultures. However, in the present study no correlation was found between the degree of c-fos induction and the extent of the maturation-enhancing stimulation. Immature cultures known to be most sensitive and responsive to these maturation-enhancing agents required relatively high doses of peptide growth factors for the induction of c-fos, and the maximal levels of c-fos mRNA elicited were much lower than those in differentiated cultures which did not show any long-term response to these stimuli.(ABSTRACT TRUNCATED AT 250 WORDS)
8-Bromo Cyclic Adenosine Monophosphate/pharmacology, Animals, Cell Differentiation, Cells, Cultured, Cytarabine/pharmacology, Diterpenes, Epidermal Growth Factor/pharmacology, Fetus, Fibroblast Growth Factor 2/pharmacology, Gene Expression/drug effects, Genes, fos/drug effects, Neurons/drug effects, Neurons/physiology, RNA/genetics, RNA/isolation & purification, RNA, Messenger/genetics, RNA, Messenger/isolation & purification, Rats, Rats, Inbred Strains, Recombinant Proteins/pharmacology, Telencephalon/embryology, Telencephalon/physiology, Terpenes/pharmacology
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