Article: article from journal or magazin.
Cysteine: depolarization-induced release from rat brain in vitro.
Journal of Neurochemistry
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Compounds released on depolarization in a Ca2+-dependent manner from rat brain slices were screened to identify candidates for neuroactive substances. Lyophilized superfusates were analyzed by reversed-phase HPLC after derivatization with 9-fluorenyl N-succinimidyl carbonate. One of the compounds that showed an increase of concentration in superfusates in the presence of iodoacetamide was identified as the cysteine (Cys) derivative, S-carboxamidomethylcysteine, by fast atom bombardment mass spectrometry and other methods. This stable Cys derivative originates from endogenous, extracellular Cys. The finding led to a method for quantification of Cys in superfusates by immediate cooling of the superfusates to 0 degrees C and reaction of Cys with N-ethylmaleimide. Depolarization-induced Ca2+-dependent release of Cys was most prominent in the neocortex, followed by the mesodiencephalon, striatum, and cerebellum. This suggests that Cys is released from a neuronal compartment and might be involved in neurotransmission.
Animals, Brain/metabolism, Brain/physiology, Chromatography, High Pressure Liquid, Cysteine/analysis, Cysteine/metabolism, Electrophysiology, Male, Rats, Rats, Inbred Strains
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