Article: article from journal or magazin.
In susceptible mice, Leishmania major induce very rapid interleukin-4 production by CD4+ T cells which are NK1.1-.
European Journal of Immunology
Susceptibility of BALB/c mice to infection with Leishmania major is associated with a T helper type 2 (Th2) response. Since interleukin-4 (IL-4) is critically required early for Th2 cell development, the kinetics of IL-4 mRNA expression was compared in susceptible and resistant mice during the first days of infection. In contrast to resistant mice, susceptible mice exhibited a peak of IL-4 mRNA in their spleens 90 min after i.v. injection of parasites and in lymph nodes 16 h after s.c. injection. IL-12 and interferon-gamma (IFN-gamma) down-regulated this early peak of IL-4 mRNA; the effect of IL-12 was IFN-gamma dependent. Treatment of resistant C57BL/6 mice with anti-IFN-gamma allowed the expression of this early IL-4 response to L. major. The increased IL-4 mRNA expression occurred in V beta 8, 7, 2- CD4+ cells in BALB/c mice and NK1.1- CD4+ cells in anti-IFN-gamma treated C57BL/6 mice. These results show that the NK1.1+ CD4+ cells, responsible for the rapid burst of IL-4 production after i.v. injection of anti-CD3, do not contribute to the early IL-4 response to L. major.
Animals, Antigens, Antigens, Ly, Antigens, Surface, CD4-Positive T-Lymphocytes/classification, CD4-Positive T-Lymphocytes/metabolism, Immunity, Innate, Immunosuppressive Agents/pharmacology, Interferon-gamma/pharmacology, Interleukin-12/pharmacology, Interleukin-4/biosynthesis, Interleukin-4/genetics, Lectins, C-Type, Leishmania major/immunology, Mice, Mice, Inbred BALB C, Mice, Inbred C3H, Mice, Inbred C57BL, Mice, Inbred CBA, Mice, Inbred DBA, NK Cell Lectin-Like Receptor Subfamily B, Neutralization Tests, Proteins, RNA, Messenger/biosynthesis, Species Specificity
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