A secreted high-affinity inhibitor of human TNF from Tanapox virus.

Détails

ID Serval
serval:BIB_7D14533F6E7F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
A secreted high-affinity inhibitor of human TNF from Tanapox virus.
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur(s)
Brunetti C.R., Paulose-Murphy M., Singh R., Qin J., Barrett J.W., Tardivel A., Schneider P., Essani K., McFadden G.
ISSN
0027-8424 (Print)
ISSN-L
0027-8424
Statut éditorial
Publié
Date de publication
2003
Volume
100
Numéro
8
Pages
4831-4836
Langue
anglais
Résumé
A class of secreted poxvirus tumor necrosis factor (TNF)-binding proteins has been isolated from Tanapox-infected cell supernatants. The inhibitor bound to a TNF-affinity column and was identified as the product of the 2L gene. Sequence analysis of 2L family members from other yatapoxviruses and swinepox virus yielded no sequence homology to any known cellular gene. The expressed Tanapox virus 2L protein bound to human TNF with high affinity (K(d) = 43 pM) and exhibits an unusually slow off-rate. However, 2L is unable to bind to a wide range of human TNF family members. The 2L protein can inhibit human TNF from binding to TNF receptors I and II as well as block TNF-induced cytolysis. Thus, Tanapox virus 2L represents an inhibitor of human TNF and offers a unique strategy with which to modulate TNF activity.
Mots-clé
Amino Acid Sequence, Animals, Antigens, CD/metabolism, Base Sequence, Carrier Proteins/genetics, Carrier Proteins/pharmacology, DNA, Viral/genetics, Genes, Viral, Humans, Mice, Molecular Sequence Data, Receptors, Tumor Necrosis Factor/metabolism, Receptors, Tumor Necrosis Factor, Type I, Receptors, Tumor Necrosis Factor, Type II, Recombinant Proteins/genetics, Recombinant Proteins/pharmacology, Sequence Homology, Amino Acid, Tumor Necrosis Factor Decoy Receptors, Tumor Necrosis Factor-alpha/antagonists &amp, inhibitors, Tumor Necrosis Factor-alpha/metabolism, Viral Proteins/genetics, Viral Proteins/pharmacology, Yaba monkey tumor virus/genetics, Yaba monkey tumor virus/physiology, Yatapoxvirus/genetics, Yatapoxvirus/physiology
Pubmed
Web of science
Open Access
Oui
Création de la notice
19/01/2008 18:30
Dernière modification de la notice
08/05/2019 20:55
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