Pulmonary delivery of cationic gold nanoparticles boost antigen-specific CD4(+) T Cell Proliferation

Details

Serval ID
serval:BIB_7C2F6498CFA7
Type
Article: article from journal or magazin.
Collection
Publications
Title
Pulmonary delivery of cationic gold nanoparticles boost antigen-specific CD4(+) T Cell Proliferation
Journal
Nanomedicine
Author(s)
Seydoux E., Rodriguez-Lorenzo L., Blom R. A., Stumbles P. A., Petri-Fink A., Rothen-Rutishauser B. M., Blank F., von Garnier C.
ISSN
1549-9642 (Electronic)
ISSN-L
1549-9634
Publication state
Published
Issued date
10/2016
Volume
12
Number
7
Pages
1815-1826
Language
english
Notes
Seydoux, Emilie
Rodriguez-Lorenzo, Laura
Blom, Rebecca A M
Stumbles, Philip A
Petri-Fink, Alke
Rothen-Rutishauser, Barbara M
Blank, Fabian
von Garnier, Christophe
eng
Nanomedicine. 2016 Oct;12(7):1815-1826. doi: 10.1016/j.nano.2016.02.020. Epub 2016 Mar 22.
Abstract
To address how surface charge affects the fate of potential nanocarriers in the lung, gold nanoparticles (AuNPs) coated with polyvinyl alcohol containing either positively (NH2) or negatively (COOH) charged functional groups were intra-nasally instilled in mice, and their uptake by antigen presenting cell populations (APC) in broncho-alveolar lavage (BAL) fluid, trachea, and lung parenchyma, as well as trafficking to the lung draining lymph nodes (LDLNs) was assessed by flow cytometry. Furthermore, CD4(+) T cell proliferation in LDLNs was investigated following instillation. All APC subpopulations preferentially captured positively-charged AuNPs compared to their negatively-charged counterparts. Uptake of AuNPs up-regulated expression of co-stimulatory molecules on all APC populations. Furthermore, positively-charged AuNPs induced enhanced OVA-specific CD4(+) T cell stimulation in LDLNs compared to negatively-charged AuNPs, or polymer alone. Our findings demonstrate surface charge as a key parameter determining particle uptake by APC, and down-stream immune responses depend on the presence of particle core-bound polymer.
Keywords
Animals, CD4-Positive T-Lymphocytes, Cell Proliferation, Gold/*administration & dosage, *Lung, *Lymphocyte Activation, Mice, *Nanoparticles, *Dendritic cell, *Gold nanoparticles, *Immune modulation, *Macrophage, *Respiratory tract
Pubmed
Create date
15/04/2021 9:58
Last modification date
01/05/2021 5:33
Usage data