Synthesis of a radioiodinated photoreactive MAGE-1 peptide derivative and photoaffinity labeling of cell-associated human leukocyte antigen-A1 molecules.

Détails

ID Serval
serval:BIB_7C255ABD58C0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Synthesis of a radioiodinated photoreactive MAGE-1 peptide derivative and photoaffinity labeling of cell-associated human leukocyte antigen-A1 molecules.
Périodique
Analytical biochemistry
Auteur(s)
Anjuère F., Layer A., Cerottini J.C., Servis C., Luescher I.F.
ISSN
0003-2697
Statut éditorial
Publié
Date de publication
1995
Peer-reviewed
Oui
Volume
229
Numéro
1
Pages
61-67
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
The synthesis of a photoreactive derivative of the human leukocyte antigen-A1 (HLA-A1)-restricted MAGE-1 peptide 161-169 (EADPTGHSY) is described. Using conventional automated solid-phase peptide synthesis, a photoreactive derivative of this peptide was synthesized by replacing histidine-167 with photo-reactive N-beta-4-azidosalicyloyl-L-2,3-diaminopropionic acid. The C-terminal tyrosine was incorporated as phosphotyrosine. This peptide derivative was radioiodinated in the presence of chloramine T. This iodination took place selectively at the photoreactive group, because the phosphate ester prevented tyrosine iodination. Following dephosphorylation with alkaline phosphatase and chromatographic purification, the radiolabeled peptide derivative was incubated with cells expressing HLA-A1 or other HLA molecules. Photoactivation resulted in efficient photoaffinity labeling of HLA-A1. Other HLA molecules or other cellular components were not detectably labeled. This labeling was inhibited by HLA-A1 but not by HLA-A2-binding peptides. This synthesis is generally applicable and can also be adapted to the synthesis of well-defined radiolabeled nonphotoreactive peptide derivatives.
Mots-clé
Affinity Labels/chemical synthesis, Affinity Labels/chemistry, Amino Acid Sequence, Antigens, Neoplasm/chemistry, Chromatography, High Pressure Liquid, HLA-A1 Antigen/analysis, Humans, Iodine Radioisotopes, Molecular Sequence Data, Neoplasm Proteins, Photochemistry
Pubmed
Web of science
Création de la notice
28/01/2008 12:19
Dernière modification de la notice
03/03/2018 18:37
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