Quantification of serotonin and eight of its metabolites in plasma of healthy volunteers by mass spectrometry.

Details

Ressource 1Download: 1-s2.0-S0009898122012633-main.pdf (818.62 [Ko])
State: Public
Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_7C0E1266F726
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Quantification of serotonin and eight of its metabolites in plasma of healthy volunteers by mass spectrometry.
Journal
Clinica chimica acta; international journal of clinical chemistry
Author(s)
Eugster P.J., Dunand M., Grund B., Ivanyuk A., Fogarasi Szabo N., Bardinet C., Abid K., Buclin T., Grouzmann E., Chtioui H.
ISSN
1873-3492 (Electronic)
ISSN-L
0009-8981
Publication state
Published
Issued date
10/08/2022
Peer-reviewed
Oui
Volume
535
Pages
19-26
Language
english
Notes
Publication types: Journal Article
Abstract
Serotonin is transformed into melatonin under the control of the light/dark cycle, representing a cornerstone of circadian rhythmicity. Serotonin also undergoes extensive metabolism to produce 5-hydroxyindoleacetic acid (5-HIAA), a biomarker for the diagnosis and monitoring of serotonin secreting neuroendocrine tumors (NETs). While serotonin, melatonin and their metabolites are part of an integrated comprehensive system, human observations about their respective plasma concentrations are still limited. We report here for the first time a multiplex UHPLC-MS/MS assay for the quantification of serotonin, 5-HIAA, 5-hydroxytryptophol (5-HTPL), N-acetyl-serotonin (NAS), Mel, 6-OH-Mel, 5-methoxytryptamine (5-MT), 5-methoxytryptophol (5-MTPL), and 5-methoxyindoleacetic acid (5-MIAA) in human plasma. Analytes were extracted by protein precipitation and solid phase extraction. Plasma concentrations for these analytes were determined in 102 healthy volunteers. The LLOQ of the assay ranges from 2.2 nM for serotonin to 1.0 pM for 6-OH-Mel. This sensitivity enables the quantification of circulating serotonin, 5-HIAA, NAS, Mel, and 5-MIAA, even at their lowest diurnal concentrations. This assay will enable specific, precise and accurate measurement of serotonin, Mel and their metabolites to draw a detailed picture of this complex pineal metabolism, allowing a dynamic understanding of these pathways and providing promising biomarkers and a metabolic signature for serotonin-secreting NETs.
Keywords
Biochemistry (medical), Clinical Biochemistry, Biochemistry, General Medicine, 5-hydroxyindoleacetic acid, LC-MS, Melatonin, Methoxyindoles, Serotonin
Pubmed
Web of science
Open Access
Yes
Create date
12/08/2022 7:21
Last modification date
01/08/2023 5:55
Usage data