Severe interference between retinal angiography and automated four-color flow cytometry analysis of blood mononuclear cells

Détails

ID Serval
serval:BIB_7BDDF03C0C67
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Severe interference between retinal angiography and automated four-color flow cytometry analysis of blood mononuclear cells
Périodique
Cytometry. Part A
Auteur(s)
Burgisser  P., Vaudaux  J., Bart  P. A.
ISSN
1552-4922 (Print)
Statut éditorial
Publié
Date de publication
08/2007
Volume
71
Numéro
8
Pages
632-6
Notes
Journal Article --- Old month value: Aug
Résumé
BACKGROUND: Retinal angiography has become a widely used diagnostic tool. It requires the intravenous administration of the fluorescent dyes fluorescein and indocyanin green. We recently received blood taken 8 h after retinal angiography, without our knowing it. We describe the failure of an automated flow cytometry system in the enumeration of lymphocyte subpopulations in this sample. METHODS: Cell enumeration was achieved by the use of the lyse-no wash MultiTEST procedure (Becton-Dickinson) together with the FACSCalibur cytometer. Absolute cell counts were obtained using TruCount beads. Data were analyzed automatically by the MultiSET and manually with the CellQuest softwares. RESULTS: The dot plots obtained with this sample looked quite abnormal. All monuclear cells stained brightly in the FITC channel irrespective of anti-CD3-FITC conjugate binding. This resulted in a major undercompensation for the increased spillover of the fluorescein emission into the PE-channel. PE-labeled cell and TruCount bead events coalesced. The MultiSET software failed to draw proper gatings and proved useless. Alternative manual gatings could partially rescue the analysis. CONCLUSIONS: Clinicians and cytometrists should be aware that, because of dye entry or binding, blood mononuclear cells collected shortly after retinal angiography are not suitable even for common cytometry applications.
Mots-clé
Automation Biological Markers/metabolism *Flow Cytometry Humans Male Middle Aged Monocytes/*cytology Retinal Vessels/*radiography T-Lymphocytes/*cytology
Pubmed
Web of science
Création de la notice
25/01/2008 15:49
Dernière modification de la notice
03/03/2018 18:36
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