Three small RNAs jointly ensure secondary metabolism and biocontrol in Pseudomonas fluorescens CHA0.

Détails

ID Serval
serval:BIB_7BB7F02593F9
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Three small RNAs jointly ensure secondary metabolism and biocontrol in Pseudomonas fluorescens CHA0.
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur(s)
Kay E., Dubuis C., Haas D.
ISSN
0027-8424[print], 0027-8424[linking]
Statut éditorial
Publié
Date de publication
2005
Volume
102
Numéro
47
Pages
17136-17141
Langue
anglais
Résumé
In many Gram-negative bacteria, the GacS/GacA two-component system positively controls the expression of extracellular products or storage compounds. In the plant-beneficial rhizosphere bacterium Pseudomonas fluorescens CHA0, the GacS/GacA system is essential for the production of antibiotic compounds and hence for biological control of root-pathogenic fungi. The small (119-nt) RNA RsmX discovered in this study, together with RsmY and RsmZ, forms a triad of GacA-dependent small RNAs, which sequester the RNA-binding proteins RsmA and RsmE and thereby antagonize translational repression exerted by these proteins in strain CHA0. This small RNA triad was found to be both necessary and sufficient for posttranscriptional derepression of biocontrol factors and for protection of cucumber from Pythium ultimum. The same three small RNAs also positively regulated swarming motility and the synthesis of a quorum-sensing signal, which is unrelated to N-acyl-homoserine lactones, and which autoinduces the Gac/Rsm cascade. Expression of RsmX and RsmY increased in parallel throughout cell growth, whereas RsmZ was produced during the late growth phase. This differential expression is assumed to facilitate fine tuning of GacS/A-controlled cell population density-dependent regulation in P. fluorescens.
Mots-clé
Bacterial Proteins/antagonists & inhibitors, Bacterial Proteins/genetics, Base Sequence, Gene Expression Regulation, Bacterial/physiology, Molecular Sequence Data, Promoter Regions, Genetic, Pseudomonas fluorescens/genetics, Pseudomonas fluorescens/growth & development, RNA Processing, Post-Transcriptional, RNA, Bacterial/genetics, RNA, Bacterial/isolation & purification, RNA, Small Cytoplasmic/genetics, RNA, Small Cytoplasmic/isolation & purification, RNA-Binding Proteins/antagonists & inhibitors, RNA-Binding Proteins/genetics, Repressor Proteins/antagonists & inhibitors, Repressor Proteins/genetics
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 18:01
Dernière modification de la notice
08/05/2019 20:50
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