Fine specificity and cross-clade reactivity of HIV type 1 Gag-specific CD4+ T cells.
Details
Serval ID
serval:BIB_7B9813F9E71B
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Fine specificity and cross-clade reactivity of HIV type 1 Gag-specific CD4+ T cells.
Journal
AIDS research and human retroviruses
ISSN
0889-2229 (Print)
ISSN-L
0889-2229
Publication state
Published
Issued date
03/2004
Peer-reviewed
Oui
Volume
20
Number
3
Pages
315-325
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Publication Status: ppublish
Abstract
Despite growing evidence that HIV-1-specific CD4(+) T helper (Th) cells may play a role in the control of viremia, discrete Th cell epitopes remain poorly defined. Furthermore, it is not known whether Th cell responses generated using vaccines based on clade B virus sequences will elicit immune responses that are effective in regions of the world where non-clade B viruses predominate. To address these issues we isolated CD4(+) T cell clones from individuals with vigorous HIV-1-specific Th cell responses and identified the minimum epitopes recognized. The minimum peptide length required for induction of CD4(+) T cell proliferation, IFN-gamma secretion, and cytolytic activity ranged from 9 to 16 amino acids in the five epitopes studied. Cross-clade recognition of the defined epitopes was examined for variant peptides from clades A, B, C, D, and AE. Over half the variant epitopes (17 of 32) exhibited impaired recognition, defined as less than 50% of the IFN-gamma secretion elicited by B clade consensus sequence. There was no evidence for antagonistic activity mediated by the variant peptides, and despite strong responses there was no escape of autologous virus from Th responses in the epitopes we studied. Abrogated recognition of variant CD4(+) T cell epitopes presents a potential obstacle to vaccine development.
Keywords
Amino Acid Sequence, CD4-Positive T-Lymphocytes/immunology, Clone Cells, Cross Reactions, Epitope Mapping, Epitopes, T-Lymphocyte/chemistry, Epitopes, T-Lymphocyte/immunology, HIV Core Protein p24/chemistry, HIV Core Protein p24/genetics, HIV Core Protein p24/immunology, HIV Infections/immunology, HIV Infections/virology, HIV-1/chemistry, HIV-1/genetics, HIV-1/immunology, Humans, Interferon-gamma/metabolism, Lymphocyte Activation, Molecular Sequence Data, Peptides/chemistry, Peptides/pharmacology
Pubmed
Web of science
Publisher's website
Create date
09/05/2023 13:00
Last modification date
29/11/2024 17:20