Selective use of TBP and TFIIB revealed by a TATA-TBP-TFIIB array with altered specificity.

Détails

ID Serval
serval:BIB_7B806C4EFE48
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Selective use of TBP and TFIIB revealed by a TATA-TBP-TFIIB array with altered specificity.
Périodique
Science
Auteur(s)
Tansey W.P., Herr W.
ISSN
0036-8075
Statut éditorial
Publié
Date de publication
02/1997
Peer-reviewed
Oui
Volume
275
Numéro
5301
Pages
829-831
Langue
anglais
Résumé
Interaction between the TATA box-binding protein TBP and TFIIB is critical for transcription in vitro. An altered-specificity TBP-TFIIB interaction was rationally designed and linked in sequence to an altered-specificity TATA box-TBP interaction to study how TBP and TFIIB function together to support transcription in human cells. The activity of this altered-specificity TATA-TBP-TFIIB array demonstrated that many activators use the known TBP-TFIIB interaction to stimulate transcription. One activator, however, derived from a glutamine-rich activation domain of Sp1, activated transcription independently of this interaction. These results reveal that selectivity in activator function in vivo can be achieved through differential use of TBP and TFIIB.
Mots-clé
DNA-Binding Proteins, Fungal Proteins, Genes, Reporter, Genes, fos, Hela Cells, Humans, Mutagenesis, Site-Directed, Sp1 Transcription Factor, TATA Box, TATA-Box Binding Protein, Trans-Activators, Transcription Factor TFIIB, Transcription Factors, Transcription, Genetic
Pubmed
Web of science
Création de la notice
24/01/2008 16:36
Dernière modification de la notice
20/08/2019 15:37
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