CD8+ T-cell protective immunity induced by immunization with Plasmodium berghei CS protein-derived synthetic peptides: evidence that localization of peptide-specific CTLs is crucial for protection against malaria

Details

Serval ID
serval:BIB_7B5E6AD78B7A
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
CD8+ T-cell protective immunity induced by immunization with Plasmodium berghei CS protein-derived synthetic peptides: evidence that localization of peptide-specific CTLs is crucial for protection against malaria
Journal
Immunology Letters
Author(s)
Renggli  J., Valmori  D., Romero  J. F., Eberl  G., Romero  P., Betschart  B., Corradin  G.
ISSN
0165-2478 (Print)
Publication state
Published
Issued date
05/1995
Volume
46
Number
1-2
Pages
199-205
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May
Abstract
Immunization of BALB/c mice (H-2d) with a mixture of major histocompatibility complex (MHC) class I- and MHC class II-restricted synthetic peptides emulsified in incomplete Freund's adjuvant (IFA) induced a high level of specific cytotoxic T lymphocyte (CTL) activity. Peptides 249-260 or 252-260, derived from the circumsporozoite protein of Plasmodium berghei and representing a H-2Kd-restricted CTL epitope, were injected twice subcutaneously or intraperitoneally in BALB/c mice in combination with the tetanus toxin-derived universal T-helper peptide P30 in IFA. No protection was observed after exposure of immunized mice to infected mosquitoes. In contrast, when peptide 252-260-specific CTLs were expanded in vitro and adoptively transferred into naive recipient, mice were partially protected (64%) against a subsequent sporozoite challenge. Furthermore, direct transfer of lymph nodes or spleen cells from mice immunized with the peptide PbCS 252-260 also conferred protection to recipient mice. This protection was long-lasting and similar to that obtained with irradiated sporozoites.
Keywords
Amino Acid Sequence Animals Epitopes Female Immunity, Cellular Malaria/immunology/*prevention & control Mice Mice, Inbred BALB C Molecular Sequence Data Peptides/chemical synthesis/*immunology Plasmodium berghei/*immunology Protozoan Proteins/*immunology T-Lymphocytes, Cytotoxic/*immunology
Pubmed
Web of science
Create date
28/01/2008 11:27
Last modification date
20/08/2019 14:37
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