Targeting TBP to a non-TATA box cis-regulatory element: a TBP-containing complex activates transcription from snRNA promoters through the PSE.

Détails

ID Serval
serval:BIB_7B526C1FCAEC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Targeting TBP to a non-TATA box cis-regulatory element: a TBP-containing complex activates transcription from snRNA promoters through the PSE.
Périodique
Genes and Development
Auteur(s)
Sadowski C.L., Henry R.W., Lobo S.M., Hernandez N.
ISSN
0890-9369 (print)
0890-9369 (linking)
Statut éditorial
Publié
Date de publication
08/1993
Volume
7
Numéro
8
Pages
1535-1548
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Résumé
In the human small nuclear RNA (snRNA) promoters, the presence of a TATA box recognized by the TATA box-binding protein (TBP) determines the selection of RNA polymerase III over RNA polymerase II. The RNA polymerase II snRNA promoters are, therefore, good candidates for TBP-independent promoters. We show here, however, that TBP activates transcription from RNA polymerase II snRNA promoters through a non-TATA box element, the snRNA proximal sequence element (PSE), as part of a new snRNA-activating protein complex (SNAPc). In contrast to the previously identified TBP-containing complexes SL1, TFIID, and TFIIIB, which appear dedicated to transcription by a single RNA polymerase, SNAPc is also essential for RNA polymerase III transcription from the U6 snRNA promoter. The U6 initiation complex appears to contain two forms of TBP, one bound to the TATA box and one bound to the PSE as a part of SNAPc, suggesting that multiple TBP molecules can have different functions within a single promoter.
Mots-clé
Animals, Base Sequence, Cross-Linking Reagents, DNA-Binding Proteins/metabolism, DNA-Directed RNA Polymerases/biosynthesis, Humans, Mice, Molecular Sequence Data, Promoter Regions, Genetic, Proteins, RNA Polymerase II/biosynthesis, RNA Polymerase III/biosynthesis, Ribonucleoprotein, U4-U6 Small Nuclear/genetics, Structure-Activity Relationship, TATA Box, TATA-Box Binding Protein, Trans-Activators/metabolism, Transcription Factors/metabolism, Transcriptional Activation
Pubmed
Web of science
Open Access
Oui
Création de la notice
21/01/2008 17:33
Dernière modification de la notice
20/08/2019 15:37
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