Effect of formate on volume reabsorption in the rabbit proximal tubule

Détails

ID Serval
serval:BIB_7B38CFD07B6D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Effect of formate on volume reabsorption in the rabbit proximal tubule
Périodique
Journal of Clinical Investigation
Auteur(s)
Schild  L., Giebisch  G., Karniski  L. P., Aronson  P. S.
ISSN
0021-9738 (Print)
Statut éditorial
Publié
Date de publication
01/1987
Volume
79
Numéro
1
Pages
32-8
Notes
In Vitro
Journal Article
Research Support, U.S. Gov't, P.H.S. --- Old month value: Jan
Résumé
Studies on microvillus membrane from rabbit kidney cortex suggest that chloride absorption may occur by chloride/formate exchange with recycling of formic acid by nonionic diffusion. We tested whether this transport mechanism participates in active NaCl reabsorption in the rabbit proximal tubule. In proximal tubule S2 segments perfused with low HCO-3 solutions, the addition of formate (0.25-0.5 mM) to the lumen and the bath increased volume reabsorption (JV) by 60%; the transepithelial potential difference remained unchanged. The effect of formate on JV was completely reversible and was inhibited both by ouabain and by luminal 4,4'-diisothiocyanostilbene-2,2'-disulfonate. Formate (0.5 mM) failed to stimulate JV in early proximal convoluted tubules perfused with high HCO-3 solutions. As measured by miniature glass pH microelectrodes, this lack of formate effect on JV was related to a less extensive acidification of the tubule fluid when high HCO-3 solutions were used as perfusate. These data suggest that chloride/formate exchange with recycling of formic acid by nonionic diffusion represents a mechanism for active, electroneutral NaCl reabsorption in the proximal tubule.
Mots-clé
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid/analogs & derivatives/pharmacology Animals Formates/*pharmacology Hydrogen-Ion Concentration Kidney Tubules, Proximal/*metabolism Ouabain/pharmacology Rabbits Sodium Chloride/metabolism Water-Electrolyte Balance/*drug effects
Pubmed
Web of science
Création de la notice
24/01/2008 13:55
Dernière modification de la notice
03/03/2018 18:35
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