Article: article from journal or magazin.
NPY, NPY receptors, and DPP IV activity are modulated by LPS, TNF-alpha and IFN-gamma in HUVEC
Journal Article Research Support, Non-U.S. Gov't --- Old month value: Nov 15
Since NPY increases endothelial cell (EC) stickiness for leukocytes, we studied the effects of LPS, TNF-alpha and IFN-gamma on its expression and action in HUVEC. Cytokines raised NPY and pro-NPY intracellular content and dipeptidyl peptidase IV (DPP IV) activity. Y1 and Y2 receptors were expressed in basal conditions, and LPS, TNF-alpha and IFN-gamma induced Y5 receptor expression with a concomitant extinction of Y2 receptor expression. NPY induced an intracellular calcium increase mainly mediated by Y2 and Y5 receptors in basal conditions. After stimulation with LPS, TNF-alpha and IFN-gamma, calcium increase was mainly caused by Y5 receptor. The modulation of the NPY system by LPS, TNF-alpha and IFN-gamma, and the NPY-induced calcium signaling suggest a role for NPY during the inflammatory response.
Antigens, CD26/*metabolism Calcium/metabolism Calcium Signaling/drug effects Cell Adhesion Molecules/genetics Gene Expression Regulation/drug effects Humans Interferon Type II/*pharmacology Lipopolysaccharides/*pharmacology Neuropeptide Y/genetics/*metabolism Receptors, Neuropeptide Y/*metabolism Tumor Necrosis Factor-alpha/*pharmacology Umbilical Cord/metabolism
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