Predominant expression of a T cell receptor V beta gene subfamily in autoimmune encephalomyelitis.

Details

Serval ID
serval:BIB_7ABE5914E496
Type
Article: article from journal or magazin.
Collection
Publications
Title
Predominant expression of a T cell receptor V beta gene subfamily in autoimmune encephalomyelitis.
Journal
Journal of Experimental Medicine
Author(s)
Zamvil S.S., Mitchell D.J., Lee N.E., Moore A.C., Waldor M.K., Sakai K., Rothbard J.B., McDevitt H.O., Steinman L., Acha-Orbea H.
ISSN
0022-1007 (Print)
ISSN-L
0022-1007
Publication state
Published
Issued date
1988
Volume
167
Number
5
Pages
1586-1596
Language
english
Abstract
TCR beta chain gene expression of individual T cell clones that share the same MHC class II restriction and similar fine specificity for the encephalitogenic NH2 terminus of the autoantigen myelin basic protein (MBP) has been examined. TCR V beta expression was examined by FACS analysis with mAbs specific for the V beta 8 subfamily of TCR beta chain genes. 14 of 18 (78%) NH2-terminal MBP-specific clones examined express a member of the TCR V beta 8 subfamily. Southern analysis was used to identify which member(s) of the TCR V beta 8 subfamily is expressed by these clones. Each of four clones examined uses V beta 8.2, though two different V beta 8.2-J beta 2 combinations were identified. Our findings indicate that there is restricted TCR V beta usage in the autoimmune T cell response to the dominant encephalitogenic NH2-terminal epitope of the MBP. The use of an mAb to the antigen-specific TCR in the prevention of T cell-mediated autoimmune disease has been investigated. Our results demonstrate that in vivo administration of a TCR V beta 8-specific mAb prevents induction of autoimmune encephalomyelitis.
Keywords
Animals, Antibodies, Monoclonal/therapeutic use, Autoimmune Diseases/genetics, Autoimmune Diseases/immunology, Encephalomyelitis, Autoimmune, Experimental/genetics, Encephalomyelitis, Autoimmune, Experimental/immunology, Female, Genes, Mice, Myelin Basic Proteins/immunology, Myelin Basic Proteins/toxicity, Receptors, Antigen, T-Cell/genetics, Receptors, Antigen, T-Cell, alpha-beta, T-Lymphocytes/immunology
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 14:48
Last modification date
20/08/2019 14:36
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