Specific targeting of human hepatocellular carcinoma cells by immunoliposomes in vitro
Details
Serval ID
serval:BIB_7ABA45D25BAC
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Specific targeting of human hepatocellular carcinoma cells by immunoliposomes in vitro
Journal
Hepatology
ISSN
0270-9139 (Print)
Publication state
Published
Issued date
11/1995
Volume
22
Number
5
Pages
1527-37
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Nov
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Nov
Abstract
The monoclonal antibody AF-20 was raised against the human hepatocellular carcinoma (HCC) cell line FOCUS and binds with high affinity to a rapidly internalized 180-kd homodimeric glycoprotein that is abundantly expressed on the surface of human HCC and other human cancer cell lines. Immunoliposomes were produced by covalently coupling AF-20 to liposomes containing carboxyfluorescein. Interaction of immunoliposomes with various HCC cell lines in vitro was quantitatively assessed by flow cytometry and qualitatively analyzed by fluorescence microscopy. Liposomes bearing an isotype-matched nonrelevant monoclonal antibody (MAb) and cell lines not expressing AF-20 antigen served as controls. AF-20-immunoliposomes specifically bound to HCC and other human cancer cell lines expressing the AF-20 antigen and were rapidly internalized at 37 degrees C. Interaction of AF-20-conjugated liposomes with these cell lines was between 5 and 200 times greater than that of unconjugated liposomes, whereas no difference was observed between control liposomes bearing a nonrelevant antibody and unconjugated liposomes. Specificity of liposome-target cell interaction was confirmed by competitive inhibition assays. Kinetic analysis showed rapid association of AF-20 immunoliposomes with target cells, with saturation conditions being reached after 60 minutes. We conclude that the MAb AF-20 directs highly efficient, specific, and rapid targeting of immunoliposomes to human HCC and other human cancer cell lines in vitro. This targeted liposomal delivery system represents a promising approach for the development of immunotargeted diagnosis and therapy strategies against HCC.
Keywords
Antibodies, Monoclonal/*administration & dosage
Antigens, Neoplasm/*immunology
Carcinoma, Hepatocellular/immunology/*therapy
Drug Carriers
Flow Cytometry
Fluorescent Dyes
Humans
Immunoconjugates
Liposomes
Liver Neoplasms/immunology/*therapy
Tumor Cells, Cultured
Pubmed
Web of science
Create date
25/01/2008 17:06
Last modification date
20/08/2019 15:36