Heterotrimeric Go protein links Wnt-Frizzled signaling with ankyrins to regulate the neuronal microtubule cytoskeleton.

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State: Public
Version: Final published version
Serval ID
serval:BIB_7A89BB65CC85
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Heterotrimeric Go protein links Wnt-Frizzled signaling with ankyrins to regulate the neuronal microtubule cytoskeleton.
Journal
Development
Author(s)
Lüchtenborg A.M., Solis G.P., Egger-Adam D., Koval A., Lin C., Blanchard M.G., Kellenberger S., Katanaev V.L.
ISSN
1477-9129 (Electronic)
ISSN-L
0950-1991
Publication state
Published
Issued date
2014
Peer-reviewed
Oui
Volume
141
Number
17
Pages
3399-3409
Language
english
Notes
Publication types: Journal Article Publication Status: ppublish
Abstract
Drosophila neuromuscular junctions (NMJs) represent a powerful model system with which to study glutamatergic synapse formation and remodeling. Several proteins have been implicated in these processes, including components of canonical Wingless (Drosophila Wnt1) signaling and the giant isoforms of the membrane-cytoskeleton linker Ankyrin 2, but possible interconnections and cooperation between these proteins were unknown. Here, we demonstrate that the heterotrimeric G protein Go functions as a transducer of Wingless-Frizzled 2 signaling in the synapse. We identify Ankyrin 2 as a target of Go signaling required for NMJ formation. Moreover, the Go-ankyrin interaction is conserved in the mammalian neurite outgrowth pathway. Without ankyrins, a major switch in the Go-induced neuronal cytoskeleton program is observed, from microtubule-dependent neurite outgrowth to actin-dependent lamellopodial induction. These findings describe a novel mechanism regulating the microtubule cytoskeleton in the nervous system. Our work in Drosophila and mammalian cells suggests that this mechanism might be generally applicable in nervous system development and function.
Pubmed
Web of science
Create date
25/09/2014 16:51
Last modification date
20/10/2020 8:19
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