Connexins 43 and 26 are differentially increased after rat bladder outlet obstruction.

Détails

ID Serval
serval:BIB_7A5F3AEC658B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Connexins 43 and 26 are differentially increased after rat bladder outlet obstruction.
Périodique
Experimental cell research
Auteur(s)
Haefliger J.A., Tissières P., Tawadros T., Formenton A., Bény J.L., Nicod P., Frey P., Meda P.
ISSN
0014-4827
Statut éditorial
Publié
Date de publication
2002
Peer-reviewed
Oui
Volume
274
Numéro
2
Pages
216-25
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Résumé
To evaluate the regulation of connexin expression by fluid pressure, we have studied the effects of elevated transmural urine pressure on Connexin43 (Cx43) and Cx26. We chose to focus on these two proteins out of the five connexins (Cx26, 43, 40, 37, and 45) which we found by RT-PCR to be expressed in the rat bladder, since in situ hybridization and immunofluorescence showed that Cx43 is the predominant connexin expressed by smooth muscle cells (SMC), whereas Cx26 is abundantly expressed only in the latter cell type. To evaluate whether these connexins are affected by changes in transmural urine pressure, we used a rat model of bladder outlet obstruction, in which a ligature is placed around the urethra. Under conditions of increased fluid pressure due to urine retention, we observed that the expression of both Cx43 and Cx26 increased at both transcript and protein levels, reaching a maximum 7-9 h after the ligature. Further analysis revealed that these changes were accounted for by a fourfold increase in Cx43 mRNA of SMC but not urothelial cell and by a fivefold increase in Cx26 mRNA of urothelium. Scrape-loading of propidium iodide showed that the latter change was paralleled by a twofold increase in coupling between urothelial cells. The data show that Cx43 and Cx26 are differentially regulated during bladder outlet obstruction and contribute to the response of the bladder wall to increased voiding pressure, possibly to control its elasticity.
Mots-clé
Animals, Cell Communication, Connexin 43, Connexins, Disease Models, Animal, Fluorescent Antibody Technique, Gap Junctions, Hypertension, Male, Muscle, Smooth, Pressure, RNA, Messenger, Rats, Rats, Wistar, Up-Regulation, Urinary Bladder, Urinary Bladder Neck Obstruction, Urothelium
Pubmed
Web of science
Création de la notice
28/02/2008 11:25
Dernière modification de la notice
20/08/2019 15:36
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