Surfactant proteins A and D enhance pulmonary clearance of Pseudomonas aeruginosa

Détails

ID Serval
serval:BIB_7A3D0E0AACA6
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Surfactant proteins A and D enhance pulmonary clearance of Pseudomonas aeruginosa
Périodique
American Journal of Respiratory Cell and Molecular Biology
Auteur(s)
Giannoni  E., Sawa  T., Allen  L., Wiener-Kronish  J., Hawgood  S.
ISSN
1044-1549 (Print)
Statut éditorial
Publié
Date de publication
06/2006
Volume
34
Numéro
6
Pages
704-10
Notes
Comparative Study
Journal Article
Research Support, N.I.H., Extramural --- Old month value: Jun
Résumé
Surfactant protein (SP)-A and SP-D, members of the collectin family, are involved in innate host defenses against various bacterial and viral pathogens. In this study, we asked whether SP-A and SP-D enhance clearance of a nonmucoid strain of Pseudomonas aeruginosa from the lungs. We infected mice deficient in SP-A (SP-A-/-), SP-D (SP-D-/-) and both pulmonary collectins (SP-AD-/-) by intratracheal administration of P. aeruginosa. Six hours after infection, bacterial counts were significantly higher in SP-A-/-, SP-D-/-, and SP-AD-/- compared with wild-type (WT) mice. Forty-eight hours after infection, bacterial counts were significantly higher in SP-A-/- mice compared with WT mice and in SP-AD-/- mice compared with WT, SP-A-/-, and SP-D-/- mice. Phagocytosis of the bacteria by alveolar macrophages was decreased in SP-A-/- and SP-D-/- mice. Levels of macrophage inflammatory peptide-2 and IL-6 were more elevated in the lungs of SP-D and SP-AD-/- mice compared with WT mice. There was more infiltration by neutrophils in the lungs of SP-D-/- compared with WT and SP-A-/- mice 48 h after infection. This study shows that SP-A and SP-D enhance pulmonary clearance of P. aeruginosa by stimulating phagocytosis by alveolar macrophages and by modulating the inflammatory response in the lungs. These findings also show that the functions of SP-A and SP-D are not completely redundant in vivo.
Mots-clé
Animals Cells, Cultured Colony Count, Microbial Interleukin-6/biosynthesis Lung/*immunology/microbiology Macrophages, Alveolar/drug effects/*immunology/microbiology Mice Mice, Inbred C57BL Mice, Knockout Monokines/biosynthesis Neutrophil Infiltration *Phagocytosis Pneumonia, Bacterial/*immunology/microbiology Pseudomonas Infections/*immunology/microbiology Pseudomonas aeruginosa/*isolation & purification Pulmonary Surfactant-Associated Protein A/genetics/immunology/*physiology Pulmonary Surfactant-Associated Protein D/genetics/immunology/*physiology Recombinant Proteins/pharmacology
Pubmed
Web of science
Création de la notice
25/01/2008 14:15
Dernière modification de la notice
03/03/2018 18:33
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