Pseudomonas aeruginosa promoters which contain a conserved GG-N10-GC motif but appear to be RpoN-independent.

Détails

ID Serval
serval:BIB_7A30FA5CB0C3
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Pseudomonas aeruginosa promoters which contain a conserved GG-N10-GC motif but appear to be RpoN-independent.
Périodique
Molecular and General Genetics
Auteur(s)
Savioz A., Zimmermann A., Haas D.
ISSN
0026-8925 (Print)
ISSN-L
0026-8925
Statut éditorial
Publié
Date de publication
1993
Volume
238
Numéro
1-2
Pages
74-80
Langue
anglais
Résumé
The proC gene of Pseudomonas aeruginosa encodes the constitutive delta 1-pyrroline 5-carboxylate reductase (the third enzyme of proline biosynthesis) and ranks among the numerous Pseudomonas genes which are poorly transcribed in Escherichia coli. The promoters of the proC gene were located by deletion mapping. The 5' ends of the proC transcripts originating from one promoter were determined by primer extension. This promoter has a GG-N10-GC motif with a 16 bp spacing between the GC doublet and the transcription start site. Such spacing is unusually long for sigma 54-dependent promoters. In rpoN mutants of P. aeruginosa and P. putida a proC'--'lacZ fusion was expressed at wild-type levels, suggesting that sigma 54 RNA polymerase is not involved in proC transcription. The expression of another P. aeruginosa gene, anr (for anaerobic regulation of nitrate respiration and anaerobic arginine degradation), also appeared to be independent of RpoN in Pseudomonas and occurred at a very low level in E. coli. The proC and anr promoters have sequence similarities in addition to the conserved GG--N10--GC motif and may also be related to some alg (alginate) promoters of P. aeruginosa. We propose that the proC and anr promoters are activated by proteins, including perhaps an alternative sigma factor, which are present in Pseudomonas but absent from E. coli.
Mots-clé
Base Sequence, Cloning, Molecular, DNA-Binding Proteins, DNA-Directed RNA Polymerases, Escherichia coli/genetics, Escherichia coli Proteins, Genes, Bacterial, Genetic Complementation Test, Genetic Markers, Molecular Sequence Data, Mutation, Plasmids, Promoter Regions, Genetic, Pseudomonas aeruginosa/enzymology, Pseudomonas aeruginosa/genetics, Pseudomonas putida/genetics, Pyrroline Carboxylate Reductases/genetics, Pyrroline Carboxylate Reductases/metabolism, RNA Polymerase Sigma 54, Recombinant Fusion Proteins/metabolism, Restriction Mapping, Sequence Deletion, Sequence Homology, Nucleic Acid, Sigma Factor/genetics, Sigma Factor/metabolism, Transcription, Genetic, beta-Galactosidase/genetics, beta-Galactosidase/metabolism
Pubmed
Web of science
Création de la notice
25/01/2008 18:01
Dernière modification de la notice
03/03/2018 18:33
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