Is Penicillin Plus Gentamicin Synergistic Against Sessile Group B Streptococcal Isolates? An <i>in Vivo</i> Study With an Experimental Model of Foreign-Body Infection.

Détails

ID Serval
serval:BIB_7A2500D146C2
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Is Penicillin Plus Gentamicin Synergistic Against Sessile Group B Streptococcal Isolates? An <i>in Vivo</i> Study With an Experimental Model of Foreign-Body Infection.
Périodique
Frontiers in microbiology
Auteur(s)
Ruppen C., Mercier T., Grandgirard D., Leib S.L., El Haj C., Murillo O., Decosterd L., Sendi P.
ISSN
1664-302X (Print)
ISSN-L
1664-302X
Statut éditorial
Publié
Date de publication
2018
Peer-reviewed
Oui
Volume
9
Pages
919
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Résumé
The rate of invasive group B <i>Streptococcus</i> (GBS) infections is steadily increasing, particularly in older persons and in adults with diabetes and other comorbidities. This population includes persons with a foreign body (e.g., who have undergone arthroplasty). In a rat tissue cage model, we evaluated the efficacy of adjunctive gentamicin (GEN) administered systemically (5 mg/kg body weight) every 24 h, or locally (12.5 mg/L tissue cage concentration) every 24 or 72 h, in combination with penicillin (PEN) administered systemically (250,000 IU/kg body weight three times per day). The efficacy was evaluated on two different sessile forms of GBS: transition (i.e., in between planktonic and biofilm) and biofilm. After 3 days of treatment, the mean bacterial load reduction of transition-form GBS was greater in all PEN-GEN combination groups than in the PEN monotherapy group ( <i>P</i> ≤ 0.03). The 6-day regimen decreased the bacterial load significantly in comparison to the 3-day regimen, irrespective of growth form and adjunctive GEN ( <i>P</i> < 0.01). After 6 days of treatment, the mean reduction in transition-form GBS was greater with PEN plus GEN administered locally every 24 h than with PEN monotherapy ( <i>P</i> = 0.03). These results were not confirmed with biofilm GBS. The difference in mean bacterial load reduction between all PEN-GEN and PEN monotherapy groups was <100 CFU/mL. Hence, synergy criteria were not fulfilled. Adjunctive systemic GEN consists of potential side effects and showed poor efficacy in this study. Combining systemic PEN and local GEN has a potential application in the treatment of streptococcal implant-associated infections.
Mots-clé
Streptococcus agalactiae, biofilms, foreign bodies, gentamicin, penicillins, synergism
Pubmed
Web of science
Création de la notice
11/06/2018 17:04
Dernière modification de la notice
16/06/2018 6:55
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