Abnormal balance between proliferation and apoptotic cell death in fibroblasts derived from keloid lesions.

Détails

ID Serval
serval:BIB_7A001F96DA63
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Abnormal balance between proliferation and apoptotic cell death in fibroblasts derived from keloid lesions.
Périodique
Plastic and reconstructive surgery
Auteur(s)
Luo S., Benathan M., Raffoul W., Panizzon R.G., Egloff D.V.
ISSN
0032-1052
Statut éditorial
Publié
Date de publication
2001
Peer-reviewed
Oui
Volume
107
Numéro
1
Pages
87-96
Langue
anglais
Notes
Publication types: Journal Article - Publication Status: ppublish
Résumé
A new culture model was developed to study the role of proliferation and apoptosis in the etiology of keloids. Fibroblasts were isolated from the superficial, central, and basal regions of six different keloid lesions by using Dulbecco's Modified Eagle Medium containing 10% fetal calf serum as a culture medium. The growth behavior of each fibroblast fraction was examined in short-term and long-term cultures, and the percentage of apoptotic cells was assessed by in situ end labeling of fragmented DNA. The fibroblasts obtained from the superficial and basal regions of keloid tissue showed population doubling times and saturation densities that were similar to those of age-matched normal fibroblasts. In contrast, the fibroblasts from the center of the keloid lesions showed significantly reduced doubling times (25.9 +/- 6.3 hours versus 43.5 +/- 6.3 hours for normal fibroblasts) and reached higher cell densities. In long-term culture, central keloid fibroblasts formed a stratified three-dimensional structure, contracted the self-produced extracellular matrix, and gave rise to nodular cell aggregates, mimicking the formation of keloid tissue. Apoptotic cells were detected in both normal and keloid-derived fibroblasts, but their numbers were twofold higher in normal cells compared with all keloid fibroblasts. To examine whether apoptosis mediates the therapeutic effect of ionizing radiation on keloids, the cells were exposed to gamma rays at a dose of 8 Gy. Under these conditions, a twofold increase in the population of apoptotic cells was detected. These results indicate that the balance between proliferation and apoptosis is impaired in keloid fibroblasts, which could be responsible for the formation of keloid tumors. The results also suggest that keloids contain at least two different fibroblast fractions that vary in growth behavior and extracellular matrix metabolism.
Mots-clé
Adult, Apoptosis, Cell Count, Cell Division, Cells, Cultured, Extracellular Matrix, Female, Fibroblasts, Gamma Rays, Humans, Keloid, Male
Pubmed
Web of science
Création de la notice
25/01/2008 17:55
Dernière modification de la notice
03/03/2018 18:33
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