Article: article from journal or magazin.
Optimal organization of a polypeptide-based candidate cancer vaccine composed of cryptic tumor peptides with enhanced immunogenicity
Journal Article --- Old month value: Mar 15
Polyspecific tumor vaccination should offer broad control of tumor cells and reduce the risk of emergence of immune escape variants. Here, we evaluated the capacity of a polypeptide composed of optimized cryptic peptides derived from three different universal tumor antigens (TERT988Y, HER-2/neu402Y and MAGE-A248V9) to induce a polyspecific CD8 cell response both in vivo in HHD mice and in vitro in humans. A mixture of TERT988Y, HER-2/neu402Y and MAGE-A248V9 peptides failed to induce a trispecific response. In contrast, a polypeptide composed of the three peptides stimulated a trispecific immune response. Interestingly, the capacity of the polypeptide to induce a trispecific response depended on its internal organization. Six different polypeptide variants corresponding to all possible combinations of the three peptides were tested. Only one variant, named Poly-6, elicited an immune response simultaneously targeting all three peptides.
Animals CD8-Positive T-Lymphocytes/immunology/metabolism Cancer Vaccines/administration & dosage/*immunology Cell Line Mice Neoplasm Proteins/administration & dosage/biosynthesis/immunology Peptide Fragments/administration & dosage/chemical synthesis/*immunology Vaccines, Combined/administration & dosage/immunology Vaccines, Subunit/administration & dosage/immunology
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