2003 Harry M. Vars Research Award. Keratinocyte growth factor improves epithelial function after massive small bowel resection.

Détails

ID Serval
serval:BIB_799019B85713
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
2003 Harry M. Vars Research Award. Keratinocyte growth factor improves epithelial function after massive small bowel resection.
Périodique
Jpen. Journal of Parenteral and Enteral Nutrition
Auteur(s)
Yang H., Wildhaber B.E., Teitelbaum D.H.
ISSN
0148-6071 (Print)
ISSN-L
0148-6071
Statut éditorial
Publié
Date de publication
2003
Volume
27
Numéro
3
Pages
198-206; discussion 206-7
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S. Publication Status: ppublish
Résumé
BACKGROUND: Massive small bowel resection with subsequent short bowel syndrome (SBS) leads to the acute loss of epithelial cell (EC) absorptive function. Keratinocyte growth factor (KGF) has been shown to improve EC growth, although little is known about KGF activity on EC function after SBS. We hypothesized that KGF would improve epithelial function in a mouse SBS model.
METHODS: Adult C57BL/6J mice were randomized to a 55% mid-small bowel resection (SBS), SBS with KGF administration (SBSKGF), or sham-operated (Control) group, and were killed at 7 days. Ussing chambers were used to study epithelial function. Short circuit current (Isc) was monitored. EC absorption was studied by measuring (1) glucose [3-O-methyl-D-[1-3H]glucose (3-OMG)] absorption; (2) sodium coupled amino acid (alanine) absorption; and (3) changes in Isc by using the absorptive agent D-glucose (stimulated Na+ absorption). Epithelial barrier function was measured with transepithelial resistance (TER) and transmural passage of 3H-mannitol (Papp). ECs were separated along the crypt-villus axis with laser capture microdissection. Epithelial KGF receptor (KGFR) mRNA expression was studied using real time reverse transcriptase-polymerase chain reaction (RT-PCR).
RESULTS: KGF administration increased the basic ion transport activity and net transepithelial absorption of 3-OMG and sodium-coupled alanine absorption. SBS significantly decreased epithelial ion transport, including the Na+ absorption stimulated by D-glucose and L-alanine. KGF administration partially improves Na+ absorption. KGF had no apparent effect on the TER and 3H-mannitol permeability in this study. KGF upregulated EC KGFR mRNA expression, predominately in the crypt and lower portion of the villus.
CONCLUSIONS: KGF administration improves epithelial absorptive function and stimulates intestinal proliferation after SBS. This suggests that KGF improves intestinal adaptation after SBS and may have clinical applicability.
Mots-clé
Animals, Epithelial Cells/metabolism, Epithelial Cells/physiology, Fibroblast Growth Factor 7, Fibroblast Growth Factors/physiology, Intestine, Small/drug effects, Intestine, Small/metabolism, Male, Mice, Mice, Inbred C57BL, Receptor, Fibroblast Growth Factor, Type 2, Receptors, Fibroblast Growth Factor/physiology, Reverse Transcriptase Polymerase Chain Reaction, Short Bowel Syndrome/surgery
Pubmed
Web of science
Création de la notice
21/02/2015 13:33
Dernière modification de la notice
03/03/2018 18:32
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