Identification of hyaluronic acid binding sites in the extracellular domain of CD44.

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State: Public
Version: Final published version
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Serval ID
serval:BIB_79660599F548
Type
Article: article from journal or magazin.
Collection
Publications
Title
Identification of hyaluronic acid binding sites in the extracellular domain of CD44.
Journal
The Journal of cell biology
Author(s)
Peach R.J., Hollenbaugh D., Stamenkovic I., Aruffo A.
ISSN
0021-9525 (Print)
ISSN-L
0021-9525
Publication state
Published
Issued date
07/1993
Peer-reviewed
Oui
Volume
122
Number
1
Pages
257-264
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
CD44 is a polymorphic glycoprotein expressed on the surface of many tissues and cell lines which has been implicated in a number of cellular functions including lymphocyte homing to mucosal lymphoid tissue (Peyers patches), leukocyte activation, lymphopoiesis, and tumor metastasis. The predominant isoform found on human leukocytes, CD44H, is a receptor for hyaluronic acid. Because of the prominent role CD44 plays in diverse biological processes, we set out to identify the hyaluronic acid binding site(s) in the extracellular domain of CD44H. Using truncation and site-directed mutagenesis we identified two regions containing clusters of conserved basic residues which are important in hyaluronic acid binding. One of these regions is situated near the NH2 terminus and is homologous to other hyaluronic acid binding proteins including cartilage link protein. The other more membrane proximal region lies outside the link protein homologous domain. Mutagenesis of basic residues within these regions established their role as determinants in hyaluronic acid binding. Mutation of Arg 41, a position where a basic residue is conserved in all hyaluronic acid binding proteins, completely abolished binding suggesting that this residue plays a critical role in hyaluronic acid binding.
Keywords
Amino Acid Sequence, Animals, Antibodies, Monoclonal/metabolism, Antigens, CD/genetics, Antigens, CD/metabolism, Base Sequence, Binding Sites, Binding Sites, Antibody, Cell Line, Cell Membrane/metabolism, Humans, Hyaluronic Acid/metabolism, Kinetics, Molecular Sequence Data, Mutagenesis, Site-Directed, Oligodeoxyribonucleotides, Receptors, Lymphocyte Homing/genetics, Receptors, Lymphocyte Homing/metabolism, Recombinant Proteins/metabolism, Transfection
Pubmed
Web of science
Open Access
Yes
Create date
29/01/2008 19:35
Last modification date
09/08/2024 13:57
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