Golgi-Resident Gαo Promotes Protrusive Membrane Dynamics.

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ID Serval
serval:BIB_794947CE9347
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Golgi-Resident Gαo Promotes Protrusive Membrane Dynamics.
Périodique
Cell
Auteur(s)
Solis G.P., Bilousov O., Koval A., Lüchtenborg A.M., Lin C., Katanaev V.L.
ISSN
1097-4172 (Electronic)
ISSN-L
0092-8674
Statut éditorial
Publié
Date de publication
24/08/2017
Peer-reviewed
Oui
Volume
170
Numéro
5
Pages
939-955.e24
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
To form protrusions like neurites, cells must coordinate their induction and growth. The first requires cytoskeletal rearrangements at the plasma membrane (PM), the second requires directed material delivery from cell's insides. We find that the Gαo-subunit of heterotrimeric G proteins localizes dually to PM and Golgi across phyla and cell types. The PM pool of Gαo induces, and the Golgi pool feeds, the growing protrusions by stimulated trafficking. Golgi-residing KDELR binds and activates monomeric Gαo, atypically for G protein-coupled receptors that normally act on heterotrimeric G proteins. Through multidimensional screenings identifying > 250 Gαo interactors, we pinpoint several basic cellular activities, including vesicular trafficking, as being regulated by Gαo. We further find small Golgi-residing GTPases Rab1 and Rab3 as direct effectors of Gαo. This KDELR → Gαo → Rab1/3 signaling axis is conserved from insects to mammals and controls material delivery from Golgi to PM in various cells and tissues.

Mots-clé
Animals, Cell Line, Cell Membrane/metabolism, Cell Surface Extensions/metabolism, Drosophila, Female, GTP Phosphohydrolases/metabolism, GTP-Binding Protein alpha Subunits, Gi-Go/metabolism, Golgi Apparatus/metabolism, Humans, Male, Mice, Mice, Inbred C57BL, Neurites/metabolism, Receptors, G-Protein-Coupled/metabolism, Receptors, Peptide/metabolism, Two-Hybrid System Techniques, rab1 GTP-Binding Proteins/metabolism, rab3 GTP-Binding Proteins/metabolism, Drosophila melanogaster, G protein coupled receptors, Golgi apparatus, Gαo interactome, KDEL receptor, Rab1/3 GTPases, heterotrimeric G proteins, neurite outgrowth, protrusion formation, vesicular trafficking
Pubmed
Web of science
Création de la notice
25/09/2017 7:29
Dernière modification de la notice
20/08/2019 14:35
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