Label-free cytotoxicity screening assay by digital holographic microscopy

Details

Serval ID
serval:BIB_793CDFD8570F
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Label-free cytotoxicity screening assay by digital holographic microscopy
Journal
Assay and Drug Development Technologies
Author(s)
Kühn J., Shaffer E., Mena J., Breton B., Parent J., Rappaz B., Chambon M., Emery Y., Magistretti P., Depeursinge C., Marquet P., Turcatti G.
ISSN
1557-8127 (Electronic)
ISSN-L
1540-658X
Publication state
Published
Issued date
2013
Volume
11
Number
2
Pages
101-107
Language
english
Notes
Publication types: Journal ArticlePublication Status: ppublish
Abstract
Abstract We introduce a label-free technology based on digital holographic microscopy (DHM) with applicability for screening by imaging, and we demonstrate its capability for cytotoxicity assessment using mammalian living cells. For this first high content screening compatible application, we automatized a digital holographic microscope for image acquisition of cells using commercially available 96-well plates. Data generated through both label-free DHM imaging and fluorescence-based methods were in good agreement for cell viability identification and a Z'-factor close to 0.9 was determined, validating the robustness of DHM assay for phenotypic screening. Further, an excellent correlation was obtained between experimental cytotoxicity dose-response curves and known IC values for different toxic compounds. For comparable results, DHM has the major advantages of being label free and close to an order of magnitude faster than automated standard fluorescence microscopy.
Pubmed
Web of science
Create date
12/04/2013 17:33
Last modification date
20/08/2019 14:35
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