Validation of methane measurement using headspace-GC-MS and quantification by a stable isotope-labeled internal standard generated in situ.

Détails

ID Serval
serval:BIB_79264189EEA6
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Validation of methane measurement using headspace-GC-MS and quantification by a stable isotope-labeled internal standard generated in situ.
Périodique
Journal of Separation Science
Auteur(s)
Varlet V., Smith F., Augsburger M.
ISSN
1615-9314 (Electronic)
ISSN-L
1615-9306
Statut éditorial
Publié
Date de publication
2013
Peer-reviewed
Oui
Volume
36
Numéro
12
Pages
1967-1972
Langue
anglais
Notes
Publication types: Journal ArticlePublication Status: ppublish
Résumé
A previous study has shown the possibility to identify methane (CH4 ) using headspace-GC-MS and quantify it with a stable isotope as internal standard. The main drawback of the GC-MS methods discussed in literature for CH4 measurement is the absence of a specific internal standard necessary to perform quantification. However, it becomes essential to develop a safer method to limit the manipulation of gaseous CH4 and to precisely control the injected amount of gas for spiking and calibration by comparison with external calibration. To avoid the manipulation of a stable isotope-labeled gas, we have chosen to generate a labeled gas as an internal standard in a vial on the basis of the formation of CH4 by the reaction of Grignard reagent methylmagnesium chloride with deuterated water. This method allows precise measurement of CH4 concentrations in gaseous sample as well as in a solid or a liquid sample after a thermodesorption step in a headspace vial. A full accuracy profile validation of this method is then presented.
Mots-clé
Deuterated methane, methane, Stable isotope-labeled standard, volatile alkane
Pubmed
Web of science
Création de la notice
18/07/2013 17:47
Dernière modification de la notice
20/08/2019 14:35
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