Negative regulation of cell-cycle progression by RINGO/Speedy E
Details
Serval ID
serval:BIB_791D7789F41C
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Negative regulation of cell-cycle progression by RINGO/Speedy E
Journal
Biochem J
ISSN
0264-6021
Publication state
Published
Issued date
2008
Volume
410
Number
3
Pages
535-42
Language
english
Notes
1470-8728
Dinarina, Ana
Ruiz, E Josué
O'Loghlen, Ana
Mouron, Silvana
Perez, Laurent
Nebreda, Angel R
Journal Article
Research Support, Non-U.S. Gov't
England
Biochem J. 2008 Mar 15;410(3):535-42. doi: 10.1042/BJ20071453.
Dinarina, Ana
Ruiz, E Josué
O'Loghlen, Ana
Mouron, Silvana
Perez, Laurent
Nebreda, Angel R
Journal Article
Research Support, Non-U.S. Gov't
England
Biochem J. 2008 Mar 15;410(3):535-42. doi: 10.1042/BJ20071453.
Abstract
Cell-cycle transitions are controlled by CDKs (cyclin-dependent kinases), whose activation is usually associated with the binding of cyclins. RINGO/Speedy proteins can also bind to and activate CDKs, although they do not have amino acid sequence homology with cyclins. The RINGO/Speedy family members studied so far positively regulate cell-cycle progression. In the present paper, we report the biochemical and functional characterization of RINGO/Speedy E. We show that RINGO/Speedy E is a functionally distant member of this protein family that negatively affects cell-cycle progression. RINGO/Speedy E overexpression inhibits the meiotic progression in Xenopus oocytes as well as the proliferation of mammalian cells. RINGO/Speedy E can bind to endogenous CDK1 and CDK2 in both cellular systems. However, the RINGO/Speedy E-activated CDKs have different substrate specificity than the CDKs activated by other RINGO/Speedy proteins, which may account for their different effects on the cell cycle. Our results indicate that, although all RINGO/Speedy family members can activate CDKs, they may differently regulate cell-cycle progression.
Keywords
Animals, Apoptosis, Blotting, Western, Cell Cycle/*physiology, Cell Cycle Proteins/metabolism/*physiology, Cell Line, Cell Proliferation, Cyclin-Dependent Kinases/metabolism, Humans, Immunoprecipitation, Substrate Specificity, Xenopus, Xenopus Proteins/metabolism/*physiology
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04/09/2020 19:03
Last modification date
07/09/2020 5:26