Measuring surface potential changes on leaves.

Détails

Ressource 1Télécharger: BIB_791806EB2CA7.P001.pdf (1974.30 [Ko])
Etat: Public
Version: Author's accepted manuscript
ID Serval
serval:BIB_791806EB2CA7
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Titre
Measuring surface potential changes on leaves.
Périodique
Nature Protocols
Auteur(s)
Mousavi S.A., Nguyen C.T., Farmer E.E., Kellenberger S.
ISSN
1750-2799 (Electronic)
ISSN-L
1750-2799
Statut éditorial
Publié
Date de publication
2014
Peer-reviewed
Oui
Volume
9
Numéro
8
Pages
1997-2004
Langue
anglais
Résumé
We provide here a detailed protocol for studying the changes in electrical surface potential of leaves. This method has been developed over the years by plant physiologists and is currently used in different variants in many laboratories. The protocol records surface potential changes to measure long-distance electrical signals induced by diverse stimuli such as leaf wounding or current injection. This technique can be used to determine signaling speeds, to measure the connectivity between different plant organs and-by exploiting mutant plants-to identify transporters and ion channels involved in electrical signaling. The approach can be combined with the analysis of mRNA expression and of metabolite concentrations to correlate electrical signaling to specific physiological events. We describe how to use this protocol on Arabidopsis, looking at the effects of leaf wounding; however, it is broadly applicable to other plants and can be used to study other aspects of plant physiology. After wound infliction, surface potential recording takes ∼20 min per plant.
Pubmed
Web of science
Open Access
Oui
Création de la notice
12/07/2014 7:51
Dernière modification de la notice
20/08/2019 14:35
Données d'usage