cDNA cloning of granzyme C, a granule-associated serine protease of cytolytic T lymphocytes

Details

Serval ID
serval:BIB_79098049474B
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
cDNA cloning of granzyme C, a granule-associated serine protease of cytolytic T lymphocytes
Journal
Journal of Immunology
Author(s)
Jenne  D., Rey  C., Masson  D., Stanley  K. K., Herz  J., Plaetinck  G., Tschopp  J.
ISSN
0022-1767 (Print)
Publication state
Published
Issued date
01/1988
Volume
140
Number
1
Pages
318-23
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jan 1
Abstract
A cDNA clone corresponding to the complete amino acid sequence of a putative protease CCP2 of murine cytotoxic T lymphocytes was isolated and sequenced. The clone encodes a 248-residue long serine esterase. The deduced N-terminal amino acid sequence is identical over 40 residues to that of granzyme C, a protease of unknown function present in granules of cytotoxic lymphocytes. Analysis of the sequence of granzyme C/CCP2 reveals high homology to other granzyme proteases, i.e. granzyme A (40%) and granzyme B (67%) and to rat mast cell protease II (46%). The amino acids lining the specificity pocket are well conserved between granzyme B, C, and rat mast cell protease II, but not granzyme A, suggesting a similar general specificity of these three proteases.
Keywords
Amino Acid Sequence Base Sequence Cloning, Molecular Cytoplasmic Granules/*enzymology DNA/genetics Granzymes Molecular Sequence Data Serine Endopeptidases/*genetics T-Lymphocytes, Cytotoxic/*enzymology
Pubmed
Web of science
Create date
24/01/2008 16:18
Last modification date
20/08/2019 15:35
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