Electron microscopy of frozen hydrated sections of vitreous ice and vitrified biological samples

Détails

ID Serval
serval:BIB_78EF7668D9D1
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Electron microscopy of frozen hydrated sections of vitreous ice and vitrified biological samples
Périodique
Journal of Microscopy
Auteur(s)
McDowall  A. W., Chang  J. J., Freeman  R., Lepault  J., Walter  C. A., Dubochet  J.
ISSN
0022-2720 (Print)
Statut éditorial
Publié
Date de publication
07/1983
Volume
131
Numéro
Pt 1
Pages
1-9
Notes
Journal Article --- Old month value: Jul
Résumé
The preparation and high resolution observation of frozen hydrated thin sections has been studied by transmission electron microscopy (TEM and STEM) on model systems, including pure water, protein solutions, catalase crystals, myelin sheath and various tissues. The state of the ice is determined by electron diffraction. Mass measurement in the electron microscope is used to determine section thickness and control hydration. An adequate depth of vitrified material for sectioning can be obtained from many biological suspensions or untreated tissues. Frozen hydrated sections around 100 nm thick can be produced under optimal conditions from vitreous ice or from vitrified biological samples. Sectioning, transfer and observation in the electron microscope is feasible without alteration of the sample hydration or its initial vitrification. Biological structures can be preserved and observed down to 10 nm. Under favourable working conditions, specimen compression during sectioning and electron beam damage are the factors limiting high resolution observations.
Mots-clé
Animals Catalase/metabolism Freezing Histological Techniques Liver/*ultrastructure Microscopy, Electron/*methods Rats
Pubmed
Web of science
Création de la notice
24/01/2008 11:25
Dernière modification de la notice
03/03/2018 18:30
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