Article: article from journal or magazin.
Case report (case report): feedback on an observation with a short commentary.
Histoplasma capsulatum var. duboisii infection in a patient with AIDS: rapid diagnosis using polymerase chain reaction-sequencing.
Diagnostic Microbiology and Infectious Disease
Publication types: Case Reports
We describe an original case of disseminated infection with Histoplasma capsulatum (Hc) var. duboisii in an African patient with AIDS who migrated to Switzerland. The diagnosis of histoplasmosis was suggested using direct examination of tissues and confirmed in 24 h with a panfungal polymerase chain reaction assay. The variety duboisii of Hc was established using DNA sequencing of the polymorphic genomic region OLE. Molecular tools allow diagnosis of histoplasmosis in 24 h, which is drastically shorter than culture procedures.
Acquired Immunodeficiency Syndrome/complications, Adult, DNA, Fungal/chemistry, DNA, Fungal/genetics, Emigrants and Immigrants, Histoplasma/genetics, Histoplasma/isolation & purification, Histoplasmosis/diagnosis, Humans, Microscopy/methods, Molecular Sequence Data, Phylogeny, Polymerase Chain Reaction/methods, Sequence Analysis, DNA, Switzerland
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