Molecular mechanisms of apoptosis in the liver of rats after portal branch ligation with and without retrorsine.

Details

Serval ID
serval:BIB_789D00B709C4
Type
Article: article from journal or magazin.
Collection
Publications
Title
Molecular mechanisms of apoptosis in the liver of rats after portal branch ligation with and without retrorsine.
Journal
Laboratory Investigation; A Journal of Technical Methods and Pathology
Author(s)
Picard C., Starkel P., Sempoux C., Saliez A., Lebrun V., Horsmans Y.
ISSN
0023-6837 (Print)
ISSN-L
0023-6837
Publication state
Published
Issued date
2004
Peer-reviewed
Oui
Volume
84
Number
5
Pages
618-628
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Abstract
The mechanisms accounting for the atrophy of the portal blood-deprived liver lobes after portal branch ligation (PBL) are still unclear. The first aim of this study was to confirm the role of apoptosis in this process and to determine which apoptotic pathways are involved. The second aim of the study was to evaluate the effect of blocking compensatory hyperplasia of the nonligated lobes with retrorsine on the mechanisms of apoptosis in the ligated lobes. Mitochondrial Bax, Bcl-2 and Bcl-X(L), cytosolic cytochrome c, caspase-3, -8 and -9 activities and TNF-alpha levels were assessed in the liver of rats before and at various time points, ranging from 30 min to 7 days, after PBL. Caspase activities were also measured in rats pretreated with retrorsine. Both the mitochondrial and the death receptor-mediated pathways are activated in the ligated liver lobes after portal branch ligation. Caspase activation is inhibited by retrorsine pretreatment, resulting in fewer apoptotic bodies. Apoptosis accounts for the atrophy of the ligated lobes after PBL. It is inhibited by retrorsine, suggesting an attempt to reduce the loss of liver mass when hyperplasia of the nonligated lobes is impaired
Keywords
Animals, Apoptosis/physiology, Atrophy, Caspase 3, Caspase 8, Caspase 9, Caspases/metabolism, Cytochromes c/metabolism, Hyperplasia, Ligation, Liver/blood supply, Liver/drug effects, Male, Organ Size/drug effects, Portal Vein/physiology, Proto-Oncogene Proteins/metabolism, Proto-Oncogene Proteins c-bcl-2/metabolism, Pyrrolizidine Alkaloids/pharmacology, Rats, Rats, Wistar, Tumor Necrosis Factor-alpha/metabolism, bcl-2-Associated X Protein, bcl-X Protein
Pubmed
Web of science
Open Access
Yes
Create date
20/10/2016 16:24
Last modification date
20/08/2019 14:35
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