A role for Yin Yang-1 (YY1) in the assembly of snRNA transcription complexes.

Détails

ID Serval
serval:BIB_77F220776836
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
A role for Yin Yang-1 (YY1) in the assembly of snRNA transcription complexes.
Périodique
Gene
Auteur(s)
Emran F., Florens L., Ma B., Swanson S.K., Washburn M.P., Hernandez N.
ISSN
0378-1119[print], 0378-1119[linking]
Statut éditorial
Publié
Date de publication
2006
Peer-reviewed
Oui
Volume
377
Pages
96-108
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
The RNA polymerase (pol) II and III human small nuclear RNA (snRNA) genes have very similar promoters and recruit a number of common factors. In particular, both types of promoters utilize the small nuclear RNA activating protein complex (SNAP(c)) and the TATA box binding protein (TBP) for basal transcription, and are activated by Oct-1. We find that SNAP(c) purified from cell lines expressing tagged SNAP(c) subunits is associated with Yin Yang-1 (YY1), a factor implicated in both activation and repression of transcription. Recombinant YY1 accelerates the binding of SNAP(c) to the proximal sequence element, its target within snRNA promoters. Moreover, it enhances the formation of a complex on the pol III U6 snRNA promoter containing all the factors (SNAP(c), TBP, TFIIB-related factor 2 (Brf2), and B double prime 1 (Bdp1)) that are sufficient to direct in vitro U6 transcription when complemented with purified pol III, as well as that of a subcomplex containing TBP, Brf2, and Bdp1. YY1 is found on both the RNA polymerase II U1 and the RNA polymerase III U6 promoters as determined by chromatin immunoprecipitations. Thus, YY1 represents a new factor that participates in transcription complexes formed on both pol II and III promoters.
Mots-clé
Animals, Binding Sites/genetics, DNA/genetics, DNA/metabolism, Hela Cells, Humans, Mice, Multiprotein Complexes, Promoter Regions, Genetic, RNA, Small Nuclear/genetics, RNA, Small Nuclear/metabolism, Recombinant Proteins/genetics, Recombinant Proteins/metabolism, Transcription Factors/genetics, Transcription Factors/metabolism, Transcription, Genetic, YY1 Transcription Factor/genetics, YY1 Transcription Factor/metabolism
Pubmed
Web of science
Création de la notice
21/01/2008 17:34
Dernière modification de la notice
20/08/2019 15:34
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