Welche Funktionen haben sechs verschiedene Gene fur sekretorische Proteinasen von Candida albicans? [What functions do six different genes for secretory proteinases have in Candida albicans?]

Détails

ID Serval
serval:BIB_77BEC5DA6BDB
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Titre
Welche Funktionen haben sechs verschiedene Gene fur sekretorische Proteinasen von Candida albicans? [What functions do six different genes for secretory proteinases have in Candida albicans?]
Périodique
Mycoses
Auteur(s)
Hube  B., Sanglard  D., Schaller  M., Ibrahim  A., Odds  F. C., Gow  N. A.
ISSN
0933-7407 (Print)
Statut éditorial
Publié
Date de publication
1998
Volume
41 Suppl 1
Pages
47-50
Notes
English Abstract
Journal Article
Review
Résumé
Secreted Aspartate Proteinases (Sap) are among those factors of the human pathogen Candida albicans, which promote infections in the immunocompromised host. Sap isoenzymes are encoded by at least nine different genes (SAP1-9), which are differentially regulated in vitro. RT-PCR analysis during experimental infections and from patient samples confirmed the expression of SAP genes in vivo. However, while Sap2 is the dominant isoenzyme under culture conditions, other SAP genes are also expressed during infections. In order to investigate the role of single isoenzymes during the pathogenesis of candidosis, mutants were produced which harbour deletions in SAP1, SAP2, SAP3 and SAP4-6. Although only SAP2 and SAP4-6 mutants showed a strong reduction of proteolytic activity in vitro, all SAP mutants were significantly attenuated in systemic infections. In addition, SAP2, SAP3 and SAP4-6 mutants were clearly more sensitive to neutrophilic leucocytes compared to the wild type SC5314. These investigations show that several proteinase isoenzymes are likely to be involved in the pathogenesis of candidosis.
Mots-clé
Candida albicans/*enzymology/*genetics/pathogenicity Candidiasis/enzymology/*etiology/genetics Endopeptidases/*genetics/physiology/secretion *Gene Expression Regulation, Fungal Humans Hydrolysis Isoenzymes/*genetics/physiology/secretion Membrane Proteins/metabolism Multigene Family Mutation Polymerase Chain Reaction Virulence
Pubmed
Web of science
Création de la notice
25/01/2008 15:40
Dernière modification de la notice
03/03/2018 18:28
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