A 7 bp mutation converts a human RNA polymerase II snRNA promoter into an RNA polymerase III promoter.

Détails

ID Serval
serval:BIB_77AC519C4BC5
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
A 7 bp mutation converts a human RNA polymerase II snRNA promoter into an RNA polymerase III promoter.
Périodique
Cell
Auteur(s)
Lobo S.M., Hernandez N.
ISSN
0092-8674[print], 0092-8674[linking]
Statut éditorial
Publié
Date de publication
07/1989
Volume
58
Numéro
1
Pages
55-67
Langue
anglais
Notes
Publication types: In Vitro ; Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Résumé
The human U2 snRNA promoter directs the formation of a specialized RNA polymerase II transcription complex that recognizes the snRNA gene 3' box as a signal for RNA 3' end formation. In contrast, the human U6 promoter is recognized by RNA polymerase III and transcription terminates in a run of Ts. We show that transcription from the U6 promoter is dependent on a sequence similar to the U2 proximal element and on an AT-rich element centered around position -27. Mutation of the AT-rich element induces RNA polymerase II transcription from the U6 promoter, whereas insertion of this element within the U2 promoter converts it into a predominantly RNA polymerase III promoter. The site of transcription termination always correlates with the nature of the transcribing polymerase: the 3' box with RNA polymerase II and a run of Ts with RNA polymerase III. Thus, a single element determines the RNA polymerase specificity of snRNA promoters and hence the site of transcription termination.
Mots-clé
Base Composition, Base Sequence, DNA Mutational Analysis, DNA-Directed RNA Polymerases/metabolism, Humans, Molecular Sequence Data, Promoter Regions, Genetic, RNA Polymerase II/metabolism, RNA Polymerase III/metabolism, RNA, Small Nuclear/genetics, Structure-Activity Relationship, Terminator Regions, Genetic, Transcription, Genetic
Pubmed
Web of science
Création de la notice
21/01/2008 16:33
Dernière modification de la notice
20/08/2019 14:34
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