Micro-patterning of biomolecules on polymer substrates.

Détails

ID Serval
serval:BIB_76E6DB210730
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Micro-patterning of biomolecules on polymer substrates.
Périodique
Langmuir
Auteur(s)
Schwarz A., Rossier J.S., Roulet E., Mermod N., Roberts M.A., Girault H.H.
ISSN
0743-7463[print], 1520-5827[electronic]
Statut éditorial
Publié
Date de publication
1998
Volume
14
Numéro
19
Pages
5526-5531
Langue
anglais
Résumé
UV−excimer laser photoablation was used, in combination with surface blocking techniques, to pattern proteins on the surfaces of polyimide and poly(ethylene terephthalate). This technique involves physical adsorption of avidin through laser-defined openings in low-temperature laminates or adsorbed protein blocking layers. Visualization of biomolecular patterns were monitored using avidin and fluorescein-labeled biotin as a model receptor−ligand couple. Adsorbed proteins could be shown to bind to UV-laser-treated polymer surfaces up to three times higher than on commercially available polymers. UV-laser photoablation was also used for the generation of three-dimensional structure, which leads to the possibility of biomolecule patterning within polymer-based microanalytical systems. The simplicity and easy handling of the described technique facilitate its application in microdiagnostic devices.
Web of science
Création de la notice
20/03/2008 13:04
Dernière modification de la notice
20/08/2019 14:34
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