Rabbit antiserum to human thymocyte membranes: specificity for normal and malignant T-lymphocytes

Détails

ID Serval
serval:BIB_76DB54FA02BF
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Rabbit antiserum to human thymocyte membranes: specificity for normal and malignant T-lymphocytes
Périodique
Clinical and Experimental Immunology
Auteur(s)
Gross  N., Bron  C.
ISSN
0009-9104
Statut éditorial
Publié
Date de publication
08/1978
Peer-reviewed
Oui
Volume
33
Numéro
2
Pages
283-91
Notes
Journal Article --- Old month value: Aug
Résumé
An antiserum was obtained by immunization of rabbits with human thymocyte membrane fractions. After appropriate absorptions, the antiserum was shown to detect specifically a population of T-cells. When tested by complement-mediated cytotoxicity the antiserum lysed 95% of thymocytes, 65% of normal PBL and 45% of tonsillar lymphocytes. It was also cytotoxic for three different T-cell lines (MOLT-4, CCRF-CEM and CCRF-HSB-2). When peripheral or tonsillar lymphocytes were separated into populations enriched in B- and T-cells, the percentage of cells lysed by the antiserum correlated well with the proportion of E-rosetting cells. Treatment of PBL with the antiserum and complement resulted in an increase of SmIg-positive B-cells in the residual cell fraction, which could no longer form E-rosettes. Treatment of PBL with the antiserum alone completely inhibited the E-rosette formation. The cytotoxic index on PBL from patients with various lymphoid disorders always correlated with the proportion of T-cells as assessed by E-rosette formation. Finally, the absorptive capacity of thymocytes for the antiserum was ten times higher as compared to that of PBL or tonsil cells.
Mots-clé
Animals Antibody Specificity *Antilymphocyte Serum B-Lymphocytes/immunology Cell Line Cell Membrane/immunology Complement System Proteins/immunology Cytotoxicity, Immunologic Humans Infant Neoplasms, Experimental/immunology Rabbits/immunology Rosette Formation T-Lymphocytes/*immunology Tonsil/immunology
Pubmed
Web of science
Création de la notice
20/01/2008 16:55
Dernière modification de la notice
03/03/2018 18:26
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