Nuclear Proteomics Uncovers Diurnal Regulatory Landscapes in Mouse Liver.

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Etat: Public
Version: Final published version
ID Serval
serval:BIB_76CD1FF92358
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Nuclear Proteomics Uncovers Diurnal Regulatory Landscapes in Mouse Liver.
Périodique
Cell Metabolism
Auteur(s)
Wang J., Mauvoisin D., Martin E., Atger F., Galindo A.N., Dayon L., Sizzano F., Palini A., Kussmann M., Waridel P., Quadroni M., Dulić V., Naef F., Gachon F.
ISSN
1932-7420 (Electronic)
ISSN-L
1550-4131
Statut éditorial
Publié
Date de publication
2017
Peer-reviewed
Oui
Volume
25
Numéro
1
Pages
102-117
Langue
anglais
Résumé
Diurnal oscillations of gene expression controlled by the circadian clock and its connected feeding rhythm enable organisms to coordinate their physiologies with daily environmental cycles. While available techniques yielded crucial insights into regulation at the transcriptional level, much less is known about temporally controlled functions within the nucleus and their regulation at the protein level. Here, we quantified the temporal nuclear accumulation of proteins and phosphoproteins from mouse liver by SILAC proteomics. We identified around 5,000 nuclear proteins, over 500 of which showed a diurnal accumulation. Parallel analysis of the nuclear phosphoproteome enabled the inference of the temporal activity of kinases accounting for rhythmic phosphorylation. Many identified rhythmic proteins were parts of nuclear complexes involved in transcriptional regulation, ribosome biogenesis, DNA repair, and the cell cycle and its potentially associated diurnal rhythm of hepatocyte polyploidy. Taken together, these findings provide unprecedented insights into the diurnal regulatory landscape of the mouse liver nucleus.

Pubmed
Web of science
Open Access
Oui
Création de la notice
30/11/2016 21:06
Dernière modification de la notice
20/08/2019 14:33
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